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TitleMechanism of actin filament severing and capping by gelsolin.
Journal, issue, pagesNat Struct Mol Biol, Vol. 32, Issue 2, Page 237-242, Year 2025
Publish dateOct 24, 2024
AuthorsKyle R Barrie / Grzegorz Rebowski / Roberto Dominguez /
PubMed AbstractGelsolin is the prototypical member of a family of Ca-activated F-actin severing and capping proteins. Here we report structures of Ca-bound human gelsolin at the barbed end of F-actin. One structure ...Gelsolin is the prototypical member of a family of Ca-activated F-actin severing and capping proteins. Here we report structures of Ca-bound human gelsolin at the barbed end of F-actin. One structure reveals gelsolin's six domains (G1G6) and interdomain linkers wrapping around F-actin, while another shows domains G1G3-a fragment observed during apoptosis-binding on both sides of F-actin. Conformational changes that trigger severing occur on one side of F-actin with G1G6 and on both sides with G1G3. Gelsolin remains bound after severing, blocking subunit exchange.
External linksNat Struct Mol Biol / PubMed:39448849 / PubMed Central
MethodsEM (single particle)
Resolution2.53 - 3.63 Å
Structure data

EMDB-43262: Consensus map of full-length gelsolin bound to the barbed end of F-actin
Method: EM (single particle) / Resolution: 2.63 Å

EMDB-43263: Local refinement of G1-G3 and F-actin barbed end subunits B-0 and B-2
Method: EM (single particle) / Resolution: 2.53 Å

EMDB-43264: Local refinement of G4-G6 and F-actin barbed end subunit B-1
Method: EM (single particle) / Resolution: 2.58 Å

EMDB-43268: Local refinement of F-actin barbed end subunits B-3, B-4, and B-5
Method: EM (single particle) / Resolution: 2.56 Å

43274

8viz

EMDB-43274, PDB-8viz:
Structure of full-length gelsolin bound to the barbed end of F-actin
Method: EM (single particle) / Resolution: 2.63 Å

43316

8vkh

EMDB-43316, PDB-8vkh:
Structure of gelsolin domains G1G3 bound to the barbed end of F-actin
Method: EM (single particle) / Resolution: 3.63 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-CA:
Unknown entry

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Source
  • homo sapiens (human)
  • oryctolagus cuniculus (rabbit)
KeywordsPROTEIN BINDING / cytoskeleton / actin / cell motility

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