EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for coupling protein transport and N-glycosylation at the mammalian endoplasmic reticulum.
ジャーナル・号・ページ	Science, Vol. 360, Issue 6385, Page 215-219, Year 2018
掲載日	2018年4月13日
著者	Katharina Braunger / Stefan Pfeffer / Shiteshu Shrimal / Reid Gilmore / Otto Berninghausen / Elisabet C Mandon / Thomas Becker / Friedrich Förster / Roland Beckmann /
PubMed 要旨	Protein synthesis, transport, and N-glycosylation are coupled at the mammalian endoplasmic reticulum by complex formation of a ribosome, the Sec61 protein-conducting channel, and ...Protein synthesis, transport, and N-glycosylation are coupled at the mammalian endoplasmic reticulum by complex formation of a ribosome, the Sec61 protein-conducting channel, and oligosaccharyltransferase (OST). Here we used different cryo-electron microscopy approaches to determine structures of native and solubilized ribosome-Sec61-OST complexes. A molecular model for the catalytic OST subunit STT3A (staurosporine and temperature sensitive 3A) revealed how it is integrated into the OST and how STT3-paralog specificity for translocon-associated OST is achieved. The OST subunit DC2 was placed at the interface between Sec61 and STT3A, where it acts as a versatile module for recruitment of STT3A-containing OST to the ribosome-Sec61 complex. This detailed structural view on the molecular architecture of the cotranslational machinery for N-glycosylation provides the basis for a mechanistic understanding of glycoprotein biogenesis at the endoplasmic reticulum.
リンク	Science / PubMed:29519914 / PubMed Central
手法	EM (サブトモグラム平均) / EM (単粒子)
解像度	4.2 - 30.0 Å
構造データ	EMDB-4306: Subtomogram average of unsorted ribosome-translocon complexes from STT3B(-/-) HEK cells 手法: EM (サブトモグラム平均) / 解像度: 30.0 Å EMDB-4307: Subtomogram average of OST-free ribosome-translocon complexes from STT3B(-/-) HEK cells 手法: EM (サブトモグラム平均) / 解像度: 30.0 Å EMDB-4308: Subtomogram average of OST-containing ribosome-translocon complexes from STT3B(-/-) HEK cells 手法: EM (サブトモグラム平均) / 解像度: 30.0 Å EMDB-4309: Subtomogram average of unsorted ribosome-translocon complexes from STT3A(-/-) HEK cells 手法: EM (サブトモグラム平均) / 解像度: 30.0 Å EMDB-4310: Subtomogram average of OST-free ribosome-translocon complexes from STT3A(-/-) HEK cells 手法: EM (サブトモグラム平均) / 解像度: 30.0 Å EMDB-4311: Subtomogram average of ribosome-translocon complexes from STT3A(-/-) HEK cells containing an unidentified ER-lumenal component 手法: EM (サブトモグラム平均) / 解像度: 30.0 Å EMDB-4312: Subtomogram average of unsorted ribosome-translocon complexes from wild type HEK cells 手法: EM (サブトモグラム平均) / 解像度: 30.0 Å EMDB-4313: Subtomogram average of OST-free ribosome-translocon complexes from wild type HEK cells 手法: EM (サブトモグラム平均) / 解像度: 30.0 Å EMDB-4314: Subtomogram average of OST-containing ribosome-translocon complexes from wild type HEK cells 手法: EM (サブトモグラム平均) / 解像度: 30.0 Å 4315 6ftg EMDB-4315, PDB-6ftg: Subtomogram average of OST-containing ribosome-translocon complexes from canine rough microsomal membranes 手法: EM (サブトモグラム平均) / 解像度: 9.1 Å 4316 6fti EMDB-4316, PDB-6fti: Cryo-EM Structure of the Mammalian Oligosaccharyltransferase Bound to Sec61 and the Programmed 80S Ribosome 手法: EM (単粒子) / 解像度: 4.2 Å 4317 6ftj EMDB-4317, PDB-6ftj: Cryo-EM Structure of the Mammalian Oligosaccharyltransferase Bound to Sec61 and the Non-programmed 80S Ribosome 手法: EM (単粒子) / 解像度: 4.7 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-9UB: [(2~{S},3~{R},4~{R},5~{S},6~{R})-3-acetamido-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-2-yl]methyl-[oxidanyl-[(2~{Z},6~{Z},10~{Z})-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraenoxy]phosphoryl]oxy-phosphinic acid
由来	Homo sapiens (ヒト) canis lupus familiaris (イヌ) oryctolagus cuniculus (ウサギ)
キーワード	PROTEIN TRANSPORT / Protein translocon of the endoplasmic reticulum