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TitleStructure and dynamics of a pentameric KCTD5/Cullin3/G beta gamma E3 ubiquitin ligase complex
Journal, issue, pagesProc. Natl. Acad. Sci. USA, Year 2024
Publish dateSep 15, 2023 (structure data deposition date)
AuthorsNguyen DM / Rath DH / Devost D / Petrin D / Rizk R / Ji AX / Narayanan N / Yong D / Zhai A / Kuntz DA ...Nguyen DM / Rath DH / Devost D / Petrin D / Rizk R / Ji AX / Narayanan N / Yong D / Zhai A / Kuntz DA / Mian MUQ / Pomroy NC / Keszei AFE / Benlekbir S / Mazhab-Jafari MT / Rubinstein JL / Hebert TE / Prive GG
External linksProc. Natl. Acad. Sci. USA / Search PubMed
MethodsEM (single particle)
Resolution2.97 - 5.7 Å
Structure data

EMDB-41994: KCTD5/Cullin3/Gbeta1gamma2 Complex: Local Refinement of KCTD5(CTD)/Gbeta1gamma2
PDB-8u7z: KCTD5/Cullin3/Gbeta1gamma2 Complex: Local Refinment of KCTD5(CTD)/Gbeta1gamma2
Method: EM (single particle) / Resolution: 2.97 Å

EMDB-41995: KCTD5/Cullin3/Gbeta1gamma2 Complex: Local Refinement of KCTD5(BTB)/Cullin3(NTD)
PDB-8u80: KCTD5/Cullin3/Gbeta1gamma2 Complex: Local Refinment of KCTD5(BTB)/Cullin3(NTD)
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-41996: KCTD5/Cullin3/Gbeta1gamma2 Complex State A: Composite Map from RELION Multi-body Refinement
PDB-8u81: KCTD5/Cullin3/Gbeta1gamma2 Complex: State A From Composite RELION Multi-body Refinement Map
Method: EM (single particle) / Resolution: 3.82 Å

EMDB-41997: KCTD5/Cullin3/Gbeta1gamma2 Complex State A: Reference Map for Composite Map EMD-41996
Method: EM (single particle) / Resolution: 3.96 Å

EMDB-41998: KCTD5/Cullin3/Gbeta1gamma2 Complex State A Body 1: RELION Multi-body Refinement of KCTD5(CTD)/Gbeta1gamma2
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-41999: KCTD5/Cullin3/Gbeta1gamma2 Complex State A Body 2: RELION Multi-body Refinemnent of KCTD5(BTB)/Cullin3(NTD)
Method: EM (single particle) / Resolution: 5.7 Å

EMDB-42000: KCTD5/Cullin3/Gbeta1gamma2 Complex State B: Composite Map from RELION Multi-body Refinement
PDB-8u82: KCTD5/Cullin3/Gbeta1gamma2 Complex: State B From Composite RELION Multi-body Refinement Map
Method: EM (single particle) / Resolution: 3.83 Å

EMDB-42001: KCTD5/Cullin3/Gbeta1gamma2 Complex State B: Reference Map for Composite Map EMD-42000
Method: EM (single particle) / Resolution: 3.96 Å

EMDB-42002: KCTD5/Cullin3/Gbeta1gamma2 Complex State B Body 1: RELION Multi-body Refinement of KCTD5(CTD)/Gbeta1gamma2
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-42003: KCTD5/Cullin3/Gbeta1gamma2 Complex State B Body 2: RELION Multi-body Refinement of KCTD5(BTB)/Cullin3(NTD)
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-42004: KCTD5/Cullin3/Gbeta1gamma2 Complex State C: Composite Map from RELION Multi-body Refinement
PDB-8u83: KCTD5/Cullin3/Gbeta1gamma2 Complex: State C From Composite RELION Multi-body Refinement Map
Method: EM (single particle) / Resolution: 3.97 Å

EMDB-42005: KCTD5/Cullin3/Gbeta1gamma2 Complex State C: Reference Map for Composite Map EMD-42004
Method: EM (single particle) / Resolution: 3.97 Å

EMDB-42006: KCTD5/Cullin3/Gbeta1gamma2 Complex State C Body 1: RELION Multi-body Refinement of KCTD5(CTD)/Gbeta1gamma2
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-42007: KCTD5/Cullin3/Gbeta1gamma2 Complex State C Body 2: RELION Multi-body Refinement of KCTD5(BTB)/Cullin3(NTD)
Method: EM (single particle) / Resolution: 4.3 Å

EMDB-42008: KCTD5/Cullin3/Gbeta1gamma2 Complex State D: Composite Map from RELION Multi-body Refinement
PDB-8u84: KCTD5/Cullin3/Gbeta1gamma2 Complex: State D From Composite RELION Multi-body Refinement Map
Method: EM (single particle) / Resolution: 3.88 Å

EMDB-42009: KCTD5/Cullin3/Gbeta1gamma2 Complex State D: Reference Map for Composite Map EMD-42008
Method: EM (single particle) / Resolution: 3.98 Å

EMDB-42010: KCTD5/Cullin3/Gbeta1gamma2 Complex State D Body 1: RELION Multi-body Refinement of KCTD5(CTD)/Gbeta1gamma2
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-42011: KCTD5/Cullin3/Gbeta1gamma2 Complex State D Body 2: RELION Multi-body Refinemnent of KCTD5(BTB)/Cullin3(NTD)
Method: EM (single particle) / Resolution: 4.2 Å

Source
  • homo sapiens (human)
KeywordsLIGASE / cullin family protein / proteasome-mediated ubiquitin-dependent protein catabolic process / complex / ubiquitin-dependent protein catabolic process

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