Steinar Halldorsson / Sai Li / Mengqiu Li / Karl Harlos / Thomas A Bowden / Juha T Huiskonen /
PubMed Abstract
Entry of enveloped viruses relies on insertion of hydrophobic residues of the viral fusion protein into the host cell membrane. However, the intermediate conformations during fusion remain unknown. ...Entry of enveloped viruses relies on insertion of hydrophobic residues of the viral fusion protein into the host cell membrane. However, the intermediate conformations during fusion remain unknown. Here, we address the fusion mechanism of Rift Valley fever virus. We determine the crystal structure of the Gn glycoprotein and fit it with the Gc fusion protein into cryo-electron microscopy reconstructions of the virion. Our analysis reveals how the Gn shields the hydrophobic fusion loops of the Gc, preventing premature fusion. Electron cryotomography of virions interacting with membranes under acidic conditions reveals how the fusogenic Gc is activated upon removal of the Gn shield. Repositioning of the Gn allows extension of Gc and insertion of fusion loops in the outer leaflet of the target membrane. These data show early structural transitions that enveloped viruses undergo during host cell entry and indicate that analogous shielding mechanisms are utilized across diverse virus families.
EMDB-4197: Icosahedral reconstruction of Rift Valley fever virus virion PDB-6f9b: Asymmetric unit of Rift Valley fever virus glycoprotein shell Method: EM (single particle) / Resolution: 13.3 Å
EMDB-4198: Localized reconstruction of the Rift Valley fever virus glycoprotein hexamer type 1 PDB-6f9c: Model of the Rift Valley fever virus glycoprotein hexamer type 1 Method: EM (single particle) / Resolution: 8.0 Å
EMDB-4199: Localized reconstruction of the Rift Valley fever virus glycoprotein hexamer type 2 PDB-6f9d: Model of the Rift Valley fever virus glycoprotein hexamer type 2 Method: EM (single particle) / Resolution: 13.3 Å
EMDB-4200: Localized reconstruction of the Rift Valley fever virus glycoprotein hexamer type 3 PDB-6f9e: Model of the Rift Valley fever virus glycoprotein hexamer type 3 Method: EM (single particle) / Resolution: 13.3 Å
EMDB-4201: Localized reconstruction of the Rift Valley fever virus glycoprotein pentamer PDB-6f9f: Model of the Rift Valley fever virus glycoprotein pentamer Method: EM (single particle) / Resolution: 13.3 Å
EMDB-4202: Subtomogram average of Rift Valley fever virus pentamer at pH 7.5 Method: EM (subtomogram averaging) / Resolution: 14.0 Å
EMDB-4203: Subtomogram average of Rift Valley fever virus hexamer at pH 7.5 Method: EM (subtomogram averaging) / Resolution: 14.0 Å
EMDB-4204: Subtomogram average of Rift Valley fever virus pentamer at pH 7.5 in absence of target membrane Method: EM (subtomogram averaging) / Resolution: 17.0 Å
EMDB-4205: Subtomogram average of Rift Valley fever virus hexamer at pH 7.5 in absence of target membrane Method: EM (subtomogram averaging) / Resolution: 14.0 Å
EMDB-4206: Subtomogram average of Rift Valley fever virus pentamer at pH 7.5 in presence of target membrane Method: EM (subtomogram averaging) / Resolution: 18.0 Å
EMDB-4207: Subtomogram average of Rift Valley fever virus hexamer at pH 7.5 in presence of target membrane Method: EM (subtomogram averaging) / Resolution: 20.0 Å
EMDB-4208: Subtomogram average of Rift Valley fever virus pentamer at pH 5.0 in absence of target membrane Method: EM (subtomogram averaging) / Resolution: 20.0 Å
EMDB-4209: Subtomogram average of Rift Valley fever virus hexamer at pH 5.0 in absence of target membrane Method: EM (subtomogram averaging) / Resolution: 16.0 Å
EMDB-4210: Subtomogram average of Rift Valley fever virus pentamer at pH 5.0 in presence of target membrane Method: EM (subtomogram averaging) / Resolution: 20.0 Å
EMDB-4211: Subtomogram average of Rift Valley fever virus hexamer at pH 5.0 in presence of target membrane Method: EM (subtomogram averaging) / Resolution: 18.0 Å
PDB-6f8p: Crystal structure of Gn from Rift Valley fever virus Method: X-RAY DIFFRACTION / Resolution: 1.6 Å
In the structure databanks used in Yorodumi, some data are registered as the other names, "COVID-19 virus" and "2019-nCoV". Here are the details of the virus and the list of structure data.
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