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TitleChallenges in making ideal cryo-EM samples.
Journal, issue, pagesCurr Opin Struct Biol, Vol. 81, Page 102646, Year 2023
Publish dateJun 29, 2023
AuthorsBong-Gyoon Han / Agustin Avila-Sakar / Jonathan Remis / Robert M Glaeser /
PubMed AbstractRecognizing that interaction with the air-water interface (AWI) is a major challenge for cryo-EM, we first review current approaches designed to avoid it. Of these, immobilizing particles on affinity ...Recognizing that interaction with the air-water interface (AWI) is a major challenge for cryo-EM, we first review current approaches designed to avoid it. Of these, immobilizing particles on affinity grids is arguably the most promising. In addition, we review efforts to gain more reliable control of the sample thicknesses, not the least important reason being to prevent immobilized particles from coming in contact with the AWI of the remaining buffer. It is emphasized that avoiding such a contact is as important for cryo-ET as for single-particle cryo-EM. Finally, looking to the future, it is proposed that immobilized samples might be used to perform time-resolved biochemical experiments directly on EM grids rather than just in test tubes or cuvettes.
External linksCurr Opin Struct Biol / PubMed:37392555
MethodsEM (single particle)
Resolution2.8 Å
Structure data

EMDB-40890: Human heavy chain apoferritin prepared with axisymmetric blotting.
Method: EM (single particle) / Resolution: 2.8 Å

Source
  • Homo sapiens (human)

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