+検索条件
-Structure paper
タイトル | Challenges in making ideal cryo-EM samples. |
---|---|
ジャーナル・号・ページ | Curr Opin Struct Biol, Vol. 81, Page 102646, Year 2023 |
掲載日 | 2023年6月29日 |
著者 | Bong-Gyoon Han / Agustin Avila-Sakar / Jonathan Remis / Robert M Glaeser / |
PubMed 要旨 | Recognizing that interaction with the air-water interface (AWI) is a major challenge for cryo-EM, we first review current approaches designed to avoid it. Of these, immobilizing particles on affinity ...Recognizing that interaction with the air-water interface (AWI) is a major challenge for cryo-EM, we first review current approaches designed to avoid it. Of these, immobilizing particles on affinity grids is arguably the most promising. In addition, we review efforts to gain more reliable control of the sample thicknesses, not the least important reason being to prevent immobilized particles from coming in contact with the AWI of the remaining buffer. It is emphasized that avoiding such a contact is as important for cryo-ET as for single-particle cryo-EM. Finally, looking to the future, it is proposed that immobilized samples might be used to perform time-resolved biochemical experiments directly on EM grids rather than just in test tubes or cuvettes. |
リンク | Curr Opin Struct Biol / PubMed:37392555 |
手法 | EM (単粒子) |
解像度 | 2.8 Å |
構造データ | EMDB-40890: Human heavy chain apoferritin prepared with axisymmetric blotting. |
由来 |
|