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-Structure paper
Title | Cellular electron cryo tomography and in situ sub-volume averaging reveal the context of microtubule-based processes. |
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Journal, issue, pages | J Struct Biol, Vol. 197, Issue 2, Page 181-190, Year 2017 |
Publish date | Jun 30, 2016 |
Authors | Michael Grange / Daven Vasishtan / Kay Grünewald / |
PubMed Abstract | Electron cryo-tomography (cryoET) is currently the only technique that allows the direct observation of proteins in their native cellular environment. Sub-volume averaging of electron tomograms ...Electron cryo-tomography (cryoET) is currently the only technique that allows the direct observation of proteins in their native cellular environment. Sub-volume averaging of electron tomograms offers a route to increase the signal-to-noise of repetitive biological structures, such improving the information content and interpretability of tomograms. We discuss the potential for sub-volume averaging in highlighting and investigating specific processes in situ, focusing on microtubule structure and viral infection. We show that (i) in situ sub-volume averaging from single tomograms can guide and complement segmentation of biological features, (ii) the in situ determination of the structure of individual viruses is possible as they infect a cell, and (iii) novel, transient processes can be imaged with high levels of detail. |
External links | J Struct Biol / PubMed:27374320 / PubMed Central |
Methods | EM (subtomogram averaging) |
Resolution | 18.5 - 25.0 Å |
Structure data | EMDB-4043: EMDB-4045: |
Source |
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