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Structure paper

TitleThe Type 9 Secretion System caught in the act of transport
Journal, issue, pagesNat Microbiol, Year 2024
Publish dateFeb 24, 2023 (structure data deposition date)
AuthorsLauber F / Deme JC / Liu X / Kjaer A / Miller HL / Alcock F / Lea SM / Berks BC
External linksSearch PubMed
MethodsEM (single particle)
Resolution2.2 - 3.2 Å
Structure data

EMDB-29911: The Type 9 Secretion System Extended Translocon - delta GldL, Peak I, SprA-PorV-RemZ-PPI focused volume
Method: EM (single particle) / Resolution: 2.4 Å

EMDB-40085: The Type 9 Secretion System Extended Translocon - delta GldL, Peak I, SprE-SkpA-Nterm SprA focused volume
Method: EM (single particle) / Resolution: 2.8 Å

EMDB-40086: The Type 9 Secretion System Extended Translocon - delta GldL, Peak I, consensus volume
Method: EM (single particle) / Resolution: 2.45 Å

40191

8gl6

EMDB-40191, PDB-8gl6:
The Type 9 Secretion System in vitro assembled, RemA-CTD substrate bound complex
Method: EM (single particle) / Resolution: 3.2 Å

40194

8gl8

EMDB-40194, PDB-8gl8:
The Type 9 Secretion System Extended Translocon - SprA-PorV-PPI-RemZ-SkpA-SprE complex
Method: EM (single particle) / Resolution: 2.2 Å

40195

8glj

EMDB-40195, PDB-8glj:
The Type 9 Secretion System in vitro assembled, FspA-CTD substrate bound complex
Method: EM (single particle) / Resolution: 3.2 Å

40196

8glk

EMDB-40196, PDB-8glk:
The Type 9 Secretion System dGldL peak II, NucA substrate bound complex
Method: EM (single particle) / Resolution: 2.8 Å

40199

8glm

EMDB-40199, PDB-8glm:
The Type 9 Secretion System in vivo assembled, RemZ substrate bound complex - conformation 1
Method: EM (single particle) / Resolution: 2.2 Å

40201

8gln

EMDB-40201, PDB-8gln:
The Type 9 Secretion System in vivo assembled, RemZ substrate bound complex - conformation 2
Method: EM (single particle) / Resolution: 2.2 Å

Chemicals

ChemComp-LMN:
Lauryl Maltose Neopentyl Glycol / detergent*YM

ChemComp-MAN:
alpha-D-mannopyranose

ChemComp-HOH:
WATER

Source
  • Flavobacterium johnsoniae UW101 (bacteria)
  • flavobacterium johnsoniae (bacteria)
KeywordsMEMBRANE PROTEIN / T9SS / Type IX Secretion System Translocon / Protein Secretion PorV - SprA - PPI - RemA Complex / Protein Secretion PorV - SprA - PPI - RemZ Complex / Protein Secretion PorV - SprA - PPI - FspA Complex / Protein Secretion PorV - SprA - PPI - NucA Complex

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