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TitleMechanistic basis for the allosteric activation of NADase activity in the Sir2-HerA antiphage defense system.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 9269, Year 2024
Publish dateOct 27, 2024
AuthorsXiangkai Zhen / Biao Zhou / Zihe Liu / Xurong Wang / Heyu Zhao / Shuxian Wu / Zekai Li / Jiamin Liang / Wanyue Zhang / Qingjian Zhu / Jun He / Xiaoli Xiong / Songying Ouyang /
PubMed AbstractSir2-HerA is a widely distributed antiphage system composed of a RecA-like ATPase (HerA) and an effector with potential NADase activity (Sir2). Sir2-HerA is believed to provide defense against phage ...Sir2-HerA is a widely distributed antiphage system composed of a RecA-like ATPase (HerA) and an effector with potential NADase activity (Sir2). Sir2-HerA is believed to provide defense against phage infection in Sir2-dependent NAD depletion to arrest the growth of infected cells. However, the detailed mechanism underlying its antiphage activity remains largely unknown. Here, we report functional investigations of Sir2-HerA from Staphylococcus aureus (SaSir2-HerA), unveiling that the NADase function of SaSir2 can be allosterically activated by the binding of SaHerA, which then assembles into a supramolecular complex with NADase activity. By combining the cryo-EM structure of SaSir2-HerA in complex with the NAD cleavage product, it is surprisingly observed that Sir2 protomers that interact with HerA are in the activated state, which is due to the opening of the α15-helix covering the active site, allowing NAD to access the catalytic pocket for hydrolysis. In brief, our study provides a comprehensive view of an allosteric activation mechanism for Sir2 NADase activity in the Sir2-HerA immune system.
External linksNat Commun / PubMed:39465277 / PubMed Central
MethodsEM (single particle)
Resolution2.8 - 2.81 Å
Structure data

39290

8yho

EMDB-39290, PDB-8yho:
Cryo-EM structure of the trimeric HerA
Method: EM (single particle) / Resolution: 2.8 Å

39302

8yhx

EMDB-39302, PDB-8yhx:
Cryo-EM structure of the trimeric HerA
Method: EM (single particle) / Resolution: 2.81 Å

Chemicals

ChemComp-APR:
ADENOSINE-5-DIPHOSPHORIBOSE

Source
  • staphylococcus aureus (bacteria)
KeywordsIMMUNE SYSTEM / antiphage system

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