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TitleCharacterization and structural analysis of a versatile aromatic prenyltransferase for imidazole-containing diketopiperazines.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 144, Year 2025
Publish dateJan 2, 2025
AuthorsWenxue Wang / Peng Wang / Chuanteng Ma / Kang Li / Zian Wang / Yuting Liu / Lu Wang / Guojian Zhang / Qian Che / Tianjiao Zhu / Yuzhong Zhang / Dehai Li /
PubMed AbstractPrenylation modifications of natural products play essential roles in chemical diversity and bioactivities, but imidazole modification prenyltransferases are not well investigated. Here, we discover ...Prenylation modifications of natural products play essential roles in chemical diversity and bioactivities, but imidazole modification prenyltransferases are not well investigated. Here, we discover a dimethylallyl tryptophan synthase family prenyltransferase, AuraA, that catalyzes the rare dimethylallylation on the imidazole moiety in the biosynthesis of aurantiamine. Biochemical assays validate that AuraA could accept both cyclo-(L-Val-L-His) and cyclo-(L-Val-DH-His) as substrates, while the prenylation modes are completely different, yielding C2-regular and C5-reverse products, respectively. Cryo-electron microscopy analysis of AuraA and its two ternary complex structures reveal two distinct modes for receptor binding, demonstrating a tolerance for altered orientations of highly similar receptors. The mutation experiments further demonstrate the promiscuity of AuraA towards imidazole-C-dimethylallylation. In this work, we also characterize a case of AuraA mutant-catalyzed dimethylallylation of imidazole moiety, offering available structural insights into the utilization and engineering of dimethylallyl tryptophan synthase family prenyltransferases.
External linksNat Commun / PubMed:39747040 / PubMed Central
MethodsEM (single particle)
Resolution2.45 - 2.95 Å
Structure data

39074

8y9d

EMDB-39074, PDB-8y9d:
Versatile Aromatic Prenyltransferase auraA in complex with DMAPP and cyclo-(L-Val-L-His)
Method: EM (single particle) / Resolution: 2.45 Å

39075

8y9e

EMDB-39075, PDB-8y9e:
Versatile Aromatic Prenyltransferase auraA for Imidazole-Containing Diketopiperazines
Method: EM (single particle) / Resolution: 2.95 Å

39079

8y9g

EMDB-39079, PDB-8y9g:
Versatile Aromatic Prenyltransferase auraA in complex with DMSPP and cyclo-(L-Val-DH-His)
Method: EM (single particle) / Resolution: 2.67 Å

61491

9jhx

EMDB-61491, PDB-9jhx:
Versatile Aromatic Prenyltransferase auraA mutant-Y207A in complex with DMSPP
Method: EM (single particle) / Resolution: 2.86 Å

Chemicals

ChemComp-DST:
DIMETHYLALLYL S-THIOLODIPHOSPHATE

PDB-1lye:
DISSECTION OF HELIX CAPPING IN T4 LYSOZYME BY STRUCTURAL AND THERMODYNAMIC ANALYSIS OF SIX AMINO ACID SUBSTITUTIONS AT THR 59

ChemComp-HOH:
WATER

PDB-1lyf:
DISSECTION OF HELIX CAPPING IN T4 LYSOZYME BY STRUCTURAL AND THERMODYNAMIC ANALYSIS OF SIX AMINO ACID SUBSTITUTIONS AT THR 59

Source
  • penicillium (fungus)
  • penicillium solitum (fungus)
KeywordsTRANSFERASE / Prenyltransferase / Imidazole-Containing Diketopiperazines

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