EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanism for the substrate recognition by a eukaryotic DNA N-adenine methyltransferase complex.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 8690, Year 2025
掲載日	2025年9月30日
著者	Qi Xu / Ying Xie / Zhubing Shi /
PubMed 要旨	In eukaryotes, DNA N-methyladenine (6mA) modification plays important roles in various cellular functions, such as chromatin dynamics, gene expression regulation, and DNA damage response. It remains ...In eukaryotes, DNA N-methyladenine (6mA) modification plays important roles in various cellular functions, such as chromatin dynamics, gene expression regulation, and DNA damage response. It remains largely unknown how eukaryotic DNA 6mA methyltransferases (MTases) recognize their substrates. Here, we reported the structures of DNA-bound eukaryotic 6mA MTase complexes. The MTA1 complex (MTA1c) in ciliates is composed of MTA1, MTA9 (or MTA9-B), p1 and p2 subunits. Cryo-electron microscopy structures of MTA1c-DNA complexes reveal that DNA lies on the surface of the MTA1-MTA9/9-B dimer and is clamped by the p1 N-terminal region. The target deoxyadenosine is flipped out of the DNA duplex and approaches the catalytic center. Unmethylated and hemi-methylated DNA substrates bind MTA1c with differential conformational dynamics. Our structural and biochemical studies shed light on the activation and substrate recognition of MTA1c and provide a framework for understanding the molecular mechanism of DNA 6mA modification in eukaryotes.
リンク	Nat Commun / PubMed:41027852 / PubMed Central
手法	EM (単粒子)
解像度	2.54 - 3.29 Å
構造データ	38777 8xyl EMDB-38777, PDB-8xyl: Cryo-EM structure of Tetrahymena DNA methyltransferase complex MTA1c 手法: EM (単粒子) / 解像度: 2.79 Å 38780 8xyp EMDB-38780, PDB-8xyp: Cryo-EM structure of SAH-bound Tetrahymena DNA methyltransferase complex MTA1c 手法: EM (単粒子) / 解像度: 2.54 Å 38781 8xyq EMDB-38781, PDB-8xyq: Cryo-EM structure of SAM-bound Tetrahymena DNA methyltransferase complex MTA1c 手法: EM (単粒子) / 解像度: 2.8 Å 38782 8xyx EMDB-38782, PDB-8xyx: Cryo-EM structure of SAM-bound Tetrahymena DNA methyltransferase complex MTA1c (D209A) 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-38786: Cryo-EM structure of DNA-bound Tetrahymena DNA methyltransferase complex MTA1c 手法: EM (単粒子) / 解像度: 3.27 Å EMDB-38787: Cryo-EM structure of DNA-bound Tetrahymena DNA methyltransferase complex MTA1c 手法: EM (単粒子) / 解像度: 3.28 Å 63956 9u92 EMDB-63956, PDB-9u92: Cryo-EM structure of Tetrahymena DNA methyltransferase complex MTA1c 手法: EM (単粒子) / 解像度: 2.68 Å 63961 9u9e EMDB-63961, PDB-9u9e: Cryo-EM structure of hemimethylated DNA-bound Tetrahymena DNA methyltransferase complex MTA1c 手法: EM (単粒子) / 解像度: 2.89 Å 63963 9vu6 EMDB-63963: Cryo-EM structure of DNA-bound Tetrahymena DNA methyltransferase complex MTA1c (MTA9-B) PDB-9vu6: Cryo-EM structure of unmethylated DNA-bound Tetrahymena DNA methyltransferase complex MTA1c (MTA9-B) 手法: EM (単粒子) / 解像度: 3.29 Å 63967 9u9k EMDB-63967, PDB-9u9k: Cryo-EM structure of hemi-methylated DNA-bound Tetrahymena DNA methyltransferase complex MTA1c (MTA9-B) 手法: EM (単粒子) / 解像度: 2.96 Å EMDB-63970: Cryo-EM structure of DNA-bound Tetrahymena DNA methyltransferase complex MTA1c 手法: EM (単粒子) / 解像度: 3.0 Å
化合物	ChemComp-SAH: S-ADENOSYL-L-HOMOCYSTEINE / S-アデノシルホモシステイン ChemComp-SAM: S-ADENOSYLMETHIONINE
由来	tetrahymena thermophila sb210 (真核生物) synthetic construct (人工物)
キーワード	DNA BINDING PROTEIN / DNA methyltransferase / DNA N6-methyladenine modification / chromatin regulation / gene regulation / protein-DNA complex / DNA BINDING PROTEIN/DNA / DNA BINDING PROTEIN-DNA complex