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-Structure paper
Title | Structure and assembly of the Ebola virus nucleocapsid. |
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Journal, issue, pages | Nature, Vol. 551, Issue 7680, Page 394-397, Year 2017 |
Publish date | Nov 16, 2017 |
Authors | William Wan / Larissa Kolesnikova / Mairi Clarke / Alexander Koehler / Takeshi Noda / Stephan Becker / John A G Briggs / |
PubMed Abstract | Ebola and Marburg viruses are filoviruses: filamentous, enveloped viruses that cause haemorrhagic fever. Filoviruses are within the order Mononegavirales, which also includes rabies virus, measles ...Ebola and Marburg viruses are filoviruses: filamentous, enveloped viruses that cause haemorrhagic fever. Filoviruses are within the order Mononegavirales, which also includes rabies virus, measles virus, and respiratory syncytial virus. Mononegaviruses have non-segmented, single-stranded negative-sense RNA genomes that are encapsidated by nucleoprotein and other viral proteins to form a helical nucleocapsid. The nucleocapsid acts as a scaffold for virus assembly and as a template for genome transcription and replication. Insights into nucleoprotein-nucleoprotein interactions have been derived from structural studies of oligomerized, RNA-encapsidating nucleoprotein, and cryo-electron microscopy of nucleocapsid or nucleocapsid-like structures. There have been no high-resolution reconstructions of complete mononegavirus nucleocapsids. Here we apply cryo-electron tomography and subtomogram averaging to determine the structure of Ebola virus nucleocapsid within intact viruses and recombinant nucleocapsid-like assemblies. These structures reveal the identity and arrangement of the nucleocapsid components, and suggest that the formation of an extended α-helix from the disordered carboxy-terminal region of nucleoprotein-core links nucleoprotein oligomerization, nucleocapsid condensation, RNA encapsidation, and accessory protein recruitment. |
External links | Nature / PubMed:29144446 / PubMed Central |
Methods | EM (subtomogram averaging) / EM (tomography) |
Resolution | 6.6 - 9.1 Å |
Structure data | EMDB-3869: The structure of Ebola virus nucleoprotein (residues 1-450) in nucleocapsid-like assemblies EMDB-3870: EMDB-3871: The structure of Ebola virus nucleocapsid-like assemblies from recombinant virus-like particles (nucleoprotein, VP24,VP35,VP40) EMDB-3872: EMDB-3873: EMDB-3874: EMDB-3875: EMDB-3876: |
Source |
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Keywords | VIRUS LIKE PARTICLE / nucleocapsid / virus-like particle |