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TitleStructural basis for activation of somatostatin receptor 5 by cyclic neuropeptide agonists.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 121, Issue 26, Page e2321710121, Year 2024
Publish dateJun 25, 2024
AuthorsJingru Li / Chongzhao You / Yang Li / Changyao Li / Wenjia Fan / Zecai Chen / Wen Hu / Kai Wu / H Eric Xu / Li-Hua Zhao /
PubMed AbstractSomatostatin receptor 5 (SSTR5) is an important G protein-coupled receptor and drug target for neuroendocrine tumors and pituitary disorders. This study presents two high-resolution cryogenicelectron ...Somatostatin receptor 5 (SSTR5) is an important G protein-coupled receptor and drug target for neuroendocrine tumors and pituitary disorders. This study presents two high-resolution cryogenicelectron microscope structures of the SSTR5-G complexes bound to the cyclic neuropeptide agonists, cortistatin-17 (CST17) and octreotide, with resolutions of 2.7 Å and 2.9 Å, respectively. The structures reveal that binding of these peptides causes rearrangement of a "hydrophobic lock", consisting of residues from transmembrane helices TM3 and TM6. This rearrangement triggers outward movement of TM6, enabling Gα protein engagement and receptor activation. In addition to hydrophobic interactions, CST17 forms conserved polar contacts similar to somatostatin-14 binding to SSTR2, while further structural and functional analysis shows that extracellular loops differently recognize CST17 and octreotide. These insights elucidate agonist selectivity and activation mechanisms of SSTR5, providing valuable guidance for structure-based drug development targeting this therapeutically relevant receptor.
External linksProc Natl Acad Sci U S A / PubMed:38885377 / PubMed Central
MethodsEM (single particle)
Resolution2.7 - 2.9 Å
Structure data

38148

8x8l

EMDB-38148, PDB-8x8l:
Cryo-EM structure of the cortistatin 17-bound Somatostatin receptor 5-Gi protein complex
Method: EM (single particle) / Resolution: 2.7 Å

38150

8x8n

EMDB-38150, PDB-8x8n:
Cryo-EM structure of the octreotide-bound Somatostatin receptor 5-Gi protein complex
Method: EM (single particle) / Resolution: 2.9 Å

Source
  • homo sapiens (human)
  • mus musculus (house mouse)
KeywordsSIGNALING PROTEIN / Cryo-EM / GPCR / Somatostatin receptor 5 / cortistatin 17 / Gi / complex / octreotide

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