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TitleIn situ architecture, function, and evolution of a contractile injection system.
Journal, issue, pagesScience, Vol. 357, Issue 6352, Page 713-717, Year 2017
Publish dateAug 18, 2017
AuthorsDésirée Böck / João M Medeiros / Han-Fei Tsao / Thomas Penz / Gregor L Weiss / Karin Aistleitner / Matthias Horn / Martin Pilhofer /
PubMed AbstractContractile injection systems mediate bacterial cell-cell interactions by a bacteriophage tail-like structure. In contrast to extracellular systems, the type 6 secretion system (T6SS) is defined by ...Contractile injection systems mediate bacterial cell-cell interactions by a bacteriophage tail-like structure. In contrast to extracellular systems, the type 6 secretion system (T6SS) is defined by intracellular localization and attachment to the cytoplasmic membrane. Here we used cryo-focused ion beam milling, electron cryotomography, and functional assays to study a T6SS in The in situ architecture revealed three modules, including a contractile sheath-tube, a baseplate, and an anchor. All modules showed conformational changes upon firing. Lateral baseplate interactions coordinated T6SSs in hexagonal arrays. The system mediated interactions with host membranes and may participate in phagosome escape. Evolutionary sequence analyses predicted that T6SSs are more widespread than previously thought. Our insights form the basis for understanding T6SS key concepts and exploring T6SS diversity.
External linksScience / PubMed:28818949 / PubMed Central
MethodsEM (tomography) / EM (subtomogram averaging)
Resolution40.0 - 60.0 Å
Structure data

EMDB-3791:
Cryotomogram of purified Amoebophilus asiaticus cells
Method: EM (tomography)

EMDB-3793:
Cryotomogram of hexagonal arrays of contractile injection systems
Method: EM (tomography)

EMDB-3794:
Thinned cryotomogram of hexagonal arrays of contractile injection systems
Method: EM (tomography)

EMDB-3795:
Subtomogram average of extended contractile injection systems
Method: EM (subtomogram averaging) / Resolution: 40.0 Å

EMDB-3796:
Subtomogram average of contracted contractile injection systems
Method: EM (subtomogram averaging) / Resolution: 60.0 Å

EMDB-3797:
Cryotomogram of intracellular amoebophili 0.25 hours post infection
Method: EM (tomography)

EMDB-3798:
Cryotomogram of intracellular amoebophili 0.5 hours post infection
Method: EM (tomography)

EMDB-3799:
Cryotomogram of intracellular amoebophili 0.75 hours post infection
Method: EM (tomography)

EMDB-3800:
Cryotomogram of intracellular amoebophili 2 hours post infection
Method: EM (tomography)

EMDB-3801:
Cryotomogram of intracellular amoebophili 1 hour post infection
Method: EM (tomography)

Source
  • Candidatus Amoebophilus asiaticus 5a2 (bacteria)

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