ジャーナル: Science / 年: 2017 タイトル: In situ architecture, function, and evolution of a contractile injection system. 著者: Désirée Böck / João M Medeiros / Han-Fei Tsao / Thomas Penz / Gregor L Weiss / Karin Aistleitner / Matthias Horn / Martin Pilhofer / 要旨: Contractile injection systems mediate bacterial cell-cell interactions by a bacteriophage tail-like structure. In contrast to extracellular systems, the type 6 secretion system (T6SS) is defined by ...Contractile injection systems mediate bacterial cell-cell interactions by a bacteriophage tail-like structure. In contrast to extracellular systems, the type 6 secretion system (T6SS) is defined by intracellular localization and attachment to the cytoplasmic membrane. Here we used cryo-focused ion beam milling, electron cryotomography, and functional assays to study a T6SS in The in situ architecture revealed three modules, including a contractile sheath-tube, a baseplate, and an anchor. All modules showed conformational changes upon firing. Lateral baseplate interactions coordinated T6SSs in hexagonal arrays. The system mediated interactions with host membranes and may participate in phagosome escape. Evolutionary sequence analyses predicted that T6SSs are more widespread than previously thought. Our insights form the basis for understanding T6SS key concepts and exploring T6SS diversity.
集束イオンビーム - 装置: OTHER / 集束イオンビーム - イオン: OTHER / 集束イオンビーム - 電圧: 30 kV / 集束イオンビーム - 電流: 0.025 nA / 集束イオンビーム - Dose rate: 1 / 集束イオンビーム - 時間: 900 sec. / 集束イオンビーム - 温度: 118 K / 集束イオンビーム - Initial thickness: 10000 / 集束イオンビーム - 最終 厚さ: 200 集束イオンビーム - 詳細: The value given for _emd_sectioning_focused_ion_beam.instrument is FEI Helios 600i. This is not in a list of allowed values set(['DB235', 'OTHER']) so OTHER is ...集束イオンビーム - 詳細: The value given for _emd_sectioning_focused_ion_beam.instrument is FEI Helios 600i. This is not in a list of allowed values set(['DB235', 'OTHER']) so OTHER is written into the XML file.
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電子顕微鏡法
顕微鏡
FEI POLARA 300
撮影
フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 平均電子線量: 1.97 e/Å2