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-Structure paper
| タイトル | In situ structural determination of cyanobacterial phycobilisome-PSII supercomplex by STAgSPA strategy. |
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| ジャーナル・号・ページ | Nat Commun, Vol. 15, Issue 1, Page 7201, Year 2024 |
| 掲載日 | 2024年8月22日 |
著者 | Xing Zhang / Yanan Xiao / Xin You / Shan Sun / Sen-Fang Sui / ![]() |
| PubMed 要旨 | Photosynthesis converting solar energy to chemical energy is one of the most important chemical reactions on earth. In cyanobacteria, light energy is captured by antenna system phycobilisomes (PBSs) ...Photosynthesis converting solar energy to chemical energy is one of the most important chemical reactions on earth. In cyanobacteria, light energy is captured by antenna system phycobilisomes (PBSs) and transferred to photosynthetic reaction centers of photosystem II (PSII) and photosystem I (PSI). While most of the protein complexes involved in photosynthesis have been characterized by in vitro structural analyses, how these protein complexes function together in vivo is not well understood. Here we implemented STAgSPA, an in situ structural analysis strategy, to solve the native structure of PBS-PSII supercomplex from the cyanobacteria Arthrospira sp. FACHB439 at resolution of ~3.5 Å. The structure reveals coupling details among adjacent PBSs and PSII dimers, and the collaborative energy transfer mechanism mediated by multiple super-PBS in cyanobacteria. Our results provide insights into the diversity of photosynthesis-related systems between prokaryotic cyanobacteria and eukaryotic red algae but are also a methodological demonstration for high-resolution structural analysis in cellular or tissue samples. |
リンク | Nat Commun / PubMed:39169020 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 3.5 - 5.1 Å |
| 構造データ | ![]() EMDB-37738: In situ PBS from cyanobacterial Spirulina platensis ![]() EMDB-37747: In situ PSII dimer from cyanobacterial Spirulina platensis EMDB-37749, PDB-8wql: ![]() EMDB-37750: In situ intact PBS-PSII supercomplex from cyanobacterial Spirulina platensis |
| 化合物 | ![]() ChemComp-CYC: ![]() ChemComp-OEX: ![]() ChemComp-CL: ![]() ChemComp-CLA: ![]() ChemComp-PL9: ![]() ChemComp-SQD: ![]() ChemComp-LMG: ![]() ChemComp-LMT: ![]() ChemComp-BCT: ![]() ChemComp-LHG: ![]() ChemComp-BCR: ![]() ChemComp-DGD: ![]() ChemComp-FE2: ![]() ChemComp-HEM: ![]() ChemComp-CA: |
| 由来 |
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キーワード | PHOTOSYNTHESIS / complex |
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Arthrospira platensis FACHB-439 (バクテリア)
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