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TitleIn situ structural determination of cyanobacterial phycobilisome-PSII supercomplex by STAgSPA strategy.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 7201, Year 2024
Publish dateAug 22, 2024
AuthorsXing Zhang / Yanan Xiao / Xin You / Shan Sun / Sen-Fang Sui /
PubMed AbstractPhotosynthesis converting solar energy to chemical energy is one of the most important chemical reactions on earth. In cyanobacteria, light energy is captured by antenna system phycobilisomes (PBSs) ...Photosynthesis converting solar energy to chemical energy is one of the most important chemical reactions on earth. In cyanobacteria, light energy is captured by antenna system phycobilisomes (PBSs) and transferred to photosynthetic reaction centers of photosystem II (PSII) and photosystem I (PSI). While most of the protein complexes involved in photosynthesis have been characterized by in vitro structural analyses, how these protein complexes function together in vivo is not well understood. Here we implemented STAgSPA, an in situ structural analysis strategy, to solve the native structure of PBS-PSII supercomplex from the cyanobacteria Arthrospira sp. FACHB439 at resolution of ~3.5 Å. The structure reveals coupling details among adjacent PBSs and PSII dimers, and the collaborative energy transfer mechanism mediated by multiple super-PBS in cyanobacteria. Our results provide insights into the diversity of photosynthesis-related systems between prokaryotic cyanobacteria and eukaryotic red algae but are also a methodological demonstration for high-resolution structural analysis in cellular or tissue samples.
External linksNat Commun / PubMed:39169020 / PubMed Central
MethodsEM (single particle)
Resolution3.5 - 5.1 Å
Structure data

EMDB-37738: In situ PBS from cyanobacterial Spirulina platensis
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-37747: In situ PSII dimer from cyanobacterial Spirulina platensis
Method: EM (single particle) / Resolution: 3.5 Å

37749

8wql

EMDB-37749, PDB-8wql:
In situ PBS-PSII supercomplex from cyanobacterial Spirulina platensis
Method: EM (single particle) / Resolution: 3.5 Å

EMDB-37750: In situ intact PBS-PSII supercomplex from cyanobacterial Spirulina platensis
Method: EM (single particle) / Resolution: 5.1 Å

Chemicals

ChemComp-CYC:
PHYCOCYANOBILIN

ChemComp-OEX:
CA-MN4-O5 CLUSTER

ChemComp-CL:
Unknown entry

ChemComp-CLA:
CHLOROPHYLL A

ChemComp-PL9:
2,3-DIMETHYL-5-(3,7,11,15,19,23,27,31,35-NONAMETHYL-2,6,10,14,18,22,26,30,34-HEXATRIACONTANONAENYL-2,5-CYCLOHEXADIENE-1,4-DIONE-2,3-DIMETHYL-5-SOLANESYL-1,4-BENZOQUINONE

ChemComp-SQD:
1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

ChemComp-BCT:
BICARBONATE ION

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM

ChemComp-BCR:
BETA-CAROTENE

ChemComp-DGD:
DIGALACTOSYL DIACYL GLYCEROL (DGDG)

ChemComp-PHO:
PHEOPHYTIN A

ChemComp-FE2:
Unknown entry

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-CA:
Unknown entry

Source
  • Arthrospira platensis FACHB-439 (bacteria)
  • Arthrospira platensis (bacteria)
  • arthrospira sp. fachb-439 (bacteria)
KeywordsPHOTOSYNTHESIS / complex

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