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TitleStructural basis of negative regulation of CRISPR-Cas7-11 by TPR-CHAT.
Journal, issue, pagesSignal Transduct Target Ther, Vol. 9, Issue 1, Page 111, Year 2024
Publish dateMay 13, 2024
AuthorsTian Hong / Qinghua Luo / Haiyun Ma / Xin Wang / Xinqiong Li / Chongrong Shen / Jie Pang / Yan Wang / Yuejia Chen / Changbin Zhang / Zhaoming Su / Haohao Dong / Xiaodi Tang /
PubMed AbstractCRISPR‒Cas7-11 is a Type III-E CRISPR-associated nuclease that functions as a potent RNA editing tool. Tetratrico-peptide repeat fused with Cas/HEF1-associated signal transducer (TPR-CHAT) acts as ...CRISPR‒Cas7-11 is a Type III-E CRISPR-associated nuclease that functions as a potent RNA editing tool. Tetratrico-peptide repeat fused with Cas/HEF1-associated signal transducer (TPR-CHAT) acts as a regulatory protein that interacts with CRISPR RNA (crRNA)-bound Cas7-11 to form a CRISPR-guided caspase complex (Craspase). However, the precise modulation of Cas7-11's nuclease activity by TPR-CHAT to enhance its utility requires further study. Here, we report cryo-electron microscopy (cryo-EM) structures of Desulfonema ishimotonii (Di) Cas7-11-crRNA, complexed with or without the full length or the N-terminus of TPR-CHAT. These structures unveil the molecular features of the Craspase complex. Structural analysis, combined with in vitro nuclease assay and electrophoretic mobility shift assay, reveals that DiTPR-CHAT negatively regulates the activity of DiCas7-11 by preventing target RNA from binding through the N-terminal 65 amino acids of DiTPR-CHAT (DiTPR-CHAT). Our work demonstrates that DiTPR-CHAT can function as a small unit of DiCas7-11 regulator, potentially enabling safe applications to prevent overcutting and off-target effects of the CRISPR‒Cas7-11 system.
External linksSignal Transduct Target Ther / PubMed:38735995 / PubMed Central
MethodsEM (single particle)
Resolution2.86 - 3.53 Å
Structure data

37640

8wm4

EMDB-37640, PDB-8wm4:
Cryo-EM structure of DiCas7-11 in complex with crRNA
Method: EM (single particle) / Resolution: 2.93 Å

37649

8wmc

EMDB-37649, PDB-8wmc:
Cryo-EM structure of DiCas7-11-crRNA in complex with regulator
Method: EM (single particle) / Resolution: 2.9 Å

37653

8wmi

EMDB-37653, PDB-8wmi:
Cryo-EM structure of DiCas7-11 mutant in complex with crRNA
Method: EM (single particle) / Resolution: 3.53 Å

37655

8wml

EMDB-37655, PDB-8wml:
Cryo-EM structure of Cas7-11-crRNA bound to N-terminal of TPR-CHAT
Method: EM (single particle) / Resolution: 2.86 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • escherichia coli (E. coli)
  • desulfonema ishimotonii (bacteria)
KeywordsIMMUNE SYSTEM / CRISPR-Cas / Gene editing / TPR-CHAT / Cas7-11

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