+検索条件
-Structure paper
| タイトル | The Eukaryotic CO-Concentrating Organelle Is Liquid-like and Exhibits Dynamic Reorganization. |
|---|---|
| ジャーナル・号・ページ | Cell, Vol. 171, Issue 1, Page 148-162.e19, Year 2017 |
| 掲載日 | 2017年9月21日 |
著者 | Elizabeth S Freeman Rosenzweig / Bin Xu / Luis Kuhn Cuellar / Antonio Martinez-Sanchez / Miroslava Schaffer / Mike Strauss / Heather N Cartwright / Pierre Ronceray / Jürgen M Plitzko / Friedrich Förster / Ned S Wingreen / Benjamin D Engel / Luke C M Mackinder / Martin C Jonikas / ![]() |
| PubMed 要旨 | Approximately 30%-40% of global CO fixation occurs inside a non-membrane-bound organelle called the pyrenoid, which is found within the chloroplasts of most eukaryotic algae. The pyrenoid matrix is ...Approximately 30%-40% of global CO fixation occurs inside a non-membrane-bound organelle called the pyrenoid, which is found within the chloroplasts of most eukaryotic algae. The pyrenoid matrix is densely packed with the CO-fixing enzyme Rubisco and is thought to be a crystalline or amorphous solid. Here, we show that the pyrenoid matrix of the unicellular alga Chlamydomonas reinhardtii is not crystalline but behaves as a liquid that dissolves and condenses during cell division. Furthermore, we show that new pyrenoids are formed both by fission and de novo assembly. Our modeling predicts the existence of a "magic number" effect associated with special, highly stable heterocomplexes that influences phase separation in liquid-like organelles. This view of the pyrenoid matrix as a phase-separated compartment provides a paradigm for understanding its structure, biogenesis, and regulation. More broadly, our findings expand our understanding of the principles that govern the architecture and inheritance of liquid-like organelles. |
リンク | Cell / PubMed:28938114 / PubMed Central |
| 手法 | EM (トモグラフィー) / EM (サブトモグラム平均) |
| 解像度 | 16.5 Å |
| 構造データ | ![]() EMDB-12749: ![]() EMDB-3694: |
| 由来 |
|
ムービー
コントローラー
構造ビューア
万見文献について



著者

リンク


