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TitleStructural insights into the substrate transport mechanism of the amino acid transporter complex.
Journal, issue, pagesJ Biol Chem, Vol. 301, Issue 9, Page 110569, Year 2025
Publish dateAug 6, 2025
AuthorsHaonan Yang / Tianhao Shi / Jing Dong / Ting Zhang / Yaning Li / Yingying Guo / Yafei Yuan / Liuqing Yang / Jin-Tang Dong / Renhong Yan /
PubMed AbstractThe -type amino acid transporter 1 (LAT1), in complex with its ancillary protein 4F2hc, mediates the sodium-independent antiport of large neutral amino acids across the plasma membrane. LAT1 ...The -type amino acid transporter 1 (LAT1), in complex with its ancillary protein 4F2hc, mediates the sodium-independent antiport of large neutral amino acids across the plasma membrane. LAT1 preferentially transports substrates, such as -leucine, -tyrosine, and -tryptophan, thyroid hormones, and drugs like 3,4-dihydroxyphenylalanine. Its pivotal role in cancer development and progression has established LAT1 as a promising therapeutic target. While prior studies have resolved the LAT1-4F2hc architecture and inhibitor interactions, the molecular basis of LAT1 substrate selectivity remains elusive. Here, we present the cryo-EM structures of LAT1-4F2hc bound to -tyrosine, -tryptophan, -leucine, and 3,4-dihydroxyphenylalanine, revealing distinct substrate binding modes. Comparative structural analysis highlights differences between LAT1 and LAT2 in substrate coordination, driven by key residues near the binding pocket that influence transport efficiency. These findings advance our mechanistic understanding of the LAT1-4F2hc complex and provide valuable insights for structure-based drug design targeting LAT1.
External linksJ Biol Chem / PubMed:40780412 / PubMed Central
MethodsEM (single particle)
Resolution3.1 - 3.58 Å
Structure data

35361

8ida

EMDB-35361, PDB-8ida:
Overall structure of the LAT1-4F2hc bound with tyrosine
Method: EM (single particle) / Resolution: 3.2 Å

36073

8j8l

EMDB-36073, PDB-8j8l:
Overall structure of the LAT1-4F2hc bound with L-dopa
Method: EM (single particle) / Resolution: 3.56 Å

36074

8j8m

EMDB-36074, PDB-8j8m:
Overall structure of the LAT1-4F2hc bound with tryptophan
Method: EM (single particle) / Resolution: 3.58 Å

37983

8x0w

EMDB-37983, PDB-8x0w:
Overall structure of the LAT1-4F2hc bound with Leu
Method: EM (single particle) / Resolution: 3.1 Å

Chemicals

ChemComp-TYR:
TYROSINE

ChemComp-DAH:
3,4-DIHYDROXYPHENYLALANINE

ChemComp-TRP:
TRYPTOPHAN

ChemComp-LEU:
LEUCINE

Source
  • homo sapiens (human)
KeywordsMEMBRANE PROTEIN / complex / amino acid

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