EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-electron microscopy structure and translocation mechanism of the crenarchaeal ribosome.
ジャーナル・号・ページ	Nucleic Acids Res, Vol. 51, Issue 17, Page 8909-8924, Year 2023
掲載日	2023年9月22日
著者	Ying-Hui Wang / Hong Dai / Ling Zhang / Yun Wu / Jingfen Wang / Chen Wang / Cai-Huang Xu / Hai Hou / Bing Yang / Yongqun Zhu / Xing Zhang / Jie Zhou /
PubMed 要旨	Archaeal ribosomes have many domain-specific features; however, our understanding of these structures is limited. We present 10 cryo-electron microscopy (cryo-EM) structures of the archaeal ribosome ...Archaeal ribosomes have many domain-specific features; however, our understanding of these structures is limited. We present 10 cryo-electron microscopy (cryo-EM) structures of the archaeal ribosome from crenarchaeota Sulfolobus acidocaldarius (Sac) at 2.7-5.7 Å resolution. We observed unstable conformations of H68 and h44 of ribosomal RNA (rRNA) in the subunit structures, which may interfere with subunit association. These subunit structures provided models for 12 rRNA expansion segments and 3 novel r-proteins. Furthermore, the 50S-aRF1 complex structure showed the unique domain orientation of aRF1, possibly explaining P-site transfer RNA (tRNA) release after translation termination. Sac 70S complexes were captured in seven distinct steps of the tRNA translocation reaction, confirming conserved structural features during archaeal ribosome translocation. In aEF2-engaged 70S ribosome complexes, 3D classification of cryo-EM data based on 30S head domain identified two new translocation intermediates with 30S head domain tilted 5-6° enabling its disengagement from the translocated tRNA and its release post-translocation. Additionally, we observed conformational changes to aEF2 during ribosome binding and switching from three different states. Our structural and biochemical data provide new insights into archaeal translation and ribosome translocation.
リンク	Nucleic Acids Res / PubMed:37604686 / PubMed Central
手法	EM (単粒子)
解像度	2.72 - 5.72 Å
構造データ	34860 8hku EMDB-34860, PDB-8hku: Cryo-EM Structures and Translocation Mechanism of Crenarchaeota Ribosome 手法: EM (単粒子) / 解像度: 2.72 Å 34861 8hkv EMDB-34861, PDB-8hkv: Cryo-EM Structures and Translocation Mechanism of Crenarchaeota Ribosome 手法: EM (単粒子) / 解像度: 4.94 Å 34862 8hkx EMDB-34862, PDB-8hkx: Cryo-EM Structures and Translocation Mechanism of Crenarchaeota Ribosome 手法: EM (単粒子) / 解像度: 3.14 Å 34863 8hky EMDB-34863, PDB-8hky: Cryo-EM Structures and Translocation Mechanism of Crenarchaeota Ribosome 手法: EM (単粒子) / 解像度: 4.45 Å 34864 8hkz EMDB-34864, PDB-8hkz: Cryo-EM Structures and Translocation Mechanism of Crenarchaeota Ribosome 手法: EM (単粒子) / 解像度: 4.78 Å 34866 8hl1 EMDB-34866, PDB-8hl1: Cryo-EM Structures and Translocation Mechanism of Crenarchaeota Ribosome 手法: EM (単粒子) / 解像度: 3.93 Å 34867 8hl2 EMDB-34867, PDB-8hl2: Cryo-EM Structures and Translocation Mechanism of Crenarchaeota Ribosome 手法: EM (単粒子) / 解像度: 4.1 Å 34868 8hl3 EMDB-34868, PDB-8hl3: Cryo-EM Structures and Translocation Mechanism of Crenarchaeota Ribosome 手法: EM (単粒子) / 解像度: 4.8 Å 34869 8hl4 EMDB-34869, PDB-8hl4: Cryo-EM Structures and Translocation Mechanism of Crenarchaeota Ribosome 手法: EM (単粒子) / 解像度: 4.62 Å 34870 8hl5 EMDB-34870, PDB-8hl5: Cryo-EM Structures and Translocation Mechanism of Crenarchaeota Ribosome 手法: EM (単粒子) / 解像度: 5.72 Å
化合物	ChemComp-UNK: UNKNOWN / (S)-2-アミノ酪酸 ChemComp-GNP: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / Gpp(NH)p / GppNHp, GMPPNP, エネルギー貯蔵分子類似体*YM / 5'-Guanylyl imidodiphosphate
由来	sulfolobus acidocaldarius dsm 639 (好気性・好酸性)
キーワード	RIBOSOME (リボソーム) / Sulfolobus acidocaldarius ribosome small subunit / Sulfolobus acidocaldarius ribosome