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TitleStructures of +1 nucleosome-bound PIC-Mediator complex.
Journal, issue, pagesScience, Vol. 378, Issue 6615, Page 62-68, Year 2022
Publish dateOct 7, 2022
AuthorsXizi Chen / Xinxin Wang / Weida Liu / Yulei Ren / Xuechun Qu / Jiabei Li / Xiaotong Yin / Yanhui Xu /
PubMed AbstractRNA polymerase II-mediated eukaryotic transcription starts with the assembly of the preinitiation complex (PIC) on core promoters. The +1 nucleosome is well positioned about 40 base pairs downstream ...RNA polymerase II-mediated eukaryotic transcription starts with the assembly of the preinitiation complex (PIC) on core promoters. The +1 nucleosome is well positioned about 40 base pairs downstream of the transcription start site (TSS) and is commonly known as a barrier of transcription. The +1 nucleosome-bound PIC-Mediator structures show that PIC-Mediator prefers binding to T40N nucleosome located 40 base pairs downstream of TSS and contacts T50N but not the T70N nucleosome. The nucleosome facilitates the organization of PIC-Mediator on the promoter by binding TFIIH subunit p52 and Mediator subunits MED19 and MED26 and may contribute to transcription initiation. PIC-Mediator exhibits multiple nucleosome-binding patterns, supporting a structural role of the +1 nucleosome in the coordination of PIC-Mediator assembly. Our study reveals the molecular mechanism of PIC-Mediator organization on chromatin and underscores the significance of the +1 nucleosome in regulating transcription initiation.
External linksScience / PubMed:36201575
MethodsEM (single particle)
Resolution4.16 - 15.18 Å
Structure data

EMDB-33867: PIC-Mediator (MED26-mut) in complex with +1 nucleosome (T40N)
Method: EM (single particle) / Resolution: 11.58 Å

EMDB-33885: PIC-Mediator (MED19-mut) in complex with +1 nucleosome (T40N)
Method: EM (single particle) / Resolution: 15.18 Å

EMDB-33886: PIC-Mediator (MED19-MED26 double mutant) in complex with +1 nucleosome (T40N)
Method: EM (single particle) / Resolution: 14.85 Å

EMDB-34215: PIC-Mediator in complex with +1 nucleosome (T50N)
Method: EM (single particle) / Resolution: 9.12 Å

EMDB-34255: PIC-Mediator (p52-mut) in complex with +1 nucleosome (T40N)
Method: EM (single particle) / Resolution: 9.9 Å

EMDB-34256: PIC-Mediator in complex with +1 H2A-containing nucleosome (T40N)
Method: EM (single particle) / Resolution: 10.84 Å

EMDB-34257: PIC-Mediator in complex with +1 nucleosome (T70N)
Method: EM (single particle) / Resolution: 5.45 Å

34359

8gxq

EMDB-34359, PDB-8gxq:
PIC-Mediator in complex with +1 nucleosome (T40N) in MH-binding state
Method: EM (single particle) / Resolution: 5.04 Å

34360

8gxs

EMDB-34360, PDB-8gxs:
PIC-Mediator in complex with +1 nucleosome (T40N) in H-binding state
Method: EM (single particle) / Resolution: 4.16 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-MG:
Unknown entry

Source
  • sus scrofa (pig)
  • synthetic construct (others)
  • homo sapiens (human)
  • xenopus laevis (African clawed frog)
KeywordsTRANSCRIPTION / PIC / Mediator / transcription initiation / +1 nucleosome

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