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TitleStructural basis for regulation of SOS response in bacteria.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 120, Issue 2, Page e2217493120, Year 2023
Publish dateJan 10, 2023
AuthorsBo Gao / Liang Liang / Lu Su / Aijia Wen / Chun Zhou / Yu Feng /
PubMed AbstractIn response to DNA damage, bacterial RecA protein forms filaments with the assistance of DinI protein. The RecA filaments stimulate the autocleavage of LexA, the repressor of more than 50 SOS genes, ...In response to DNA damage, bacterial RecA protein forms filaments with the assistance of DinI protein. The RecA filaments stimulate the autocleavage of LexA, the repressor of more than 50 SOS genes, and activate the SOS response. During the late phase of SOS response, the RecA filaments stimulate the autocleavage of UmuD and λ repressor CI, leading to mutagenic repair and lytic cycle, respectively. Here, we determined the cryo-electron microscopy structures of RecA filaments in complex with DinI, LexA, UmuD, and λCI by helical reconstruction. The structures reveal that LexA and UmuD dimers bind in the filament groove and cleave in an intramolecular and an intermolecular manner, respectively, while λCI binds deeply in the filament groove as a monomer. Despite their distinct folds and oligomeric states, all RecA filament binders recognize the same conserved protein features in the filament groove. The SOS response in bacteria can lead to mutagenesis and antimicrobial resistance, and our study paves the way for rational drug design targeting the bacterial SOS response.
External linksProc Natl Acad Sci U S A / PubMed:36598938 / PubMed Central
MethodsEM (helical sym.)
Resolution2.78 - 3.31 Å
Structure data

34151

7ywa

EMDB-34151, PDB-7ywa:
Structure of DinI in complex with RecA filament
Method: EM (helical sym.) / Resolution: 3.26 Å

34152

8gms

EMDB-34152, PDB-8gms:
Structure of LexA in complex with RecA filament
Method: EM (helical sym.) / Resolution: 3.31 Å

34153

8gmt

EMDB-34153, PDB-8gmt:
Structure of UmuD in complex with RecA filament
Method: EM (helical sym.) / Resolution: 3.31 Å

34154

8gmu

EMDB-34154, PDB-8gmu:
Structure of lambda repressor in complex with RecA filament
Method: EM (helical sym.) / Resolution: 2.78 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-AGS:
PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-gamma-S, energy-carrying molecule analogue*YM

Source
  • escherichia coli (E. coli)
  • escherichia phage lambda (virus)
KeywordsDNA BINDING PROTEIN/DNA / SOS response / Filament / DNA repair / DNA BINDING PROTEIN-DNA COMPLEX / Helical reconstruction / RecA / UmuD / lambda repressor

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