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TitleMolecular mechanism of antibody neutralization of coxsackievirus A16.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 7854, Year 2022
Publish dateDec 21, 2022
AuthorsChao Zhang / Caixuan Liu / Jinping Shi / Yalei Wang / Cong Xu / Xiaohua Ye / Qingwei Liu / Xue Li / Weihua Qiao / Yannan Yin / Yao Cong / Zhong Huang /
PubMed AbstractCoxsackievirus A16 (CVA16) causes hand, foot and mouth disease in infants and young children. However, no vaccine or anti-viral agent is currently available for CVA16. Here, the functions and working ...Coxsackievirus A16 (CVA16) causes hand, foot and mouth disease in infants and young children. However, no vaccine or anti-viral agent is currently available for CVA16. Here, the functions and working mechanisms of two CVA16-specific neutralizing monoclonal antibodies (MAbs), 9B5 and 8C4, are comprehensively investigated. Both 9B5 and 8C4 display potent neutralization in vitro and prophylactic and therapeutic efficacy in a mouse model of CVA16 infection. Mechanistically, 9B5 exerts neutralization primarily through inhibiting CVA16 attachment to cell surface via blockade of CVA16 binding to its attachment receptor, heparan sulfate, whereas 8C4 functions mainly at the post-attachment stage of CVA16 entry by interfering with the interaction between CVA16 and its uncoating receptor SCARB2. Cryo-EM studies show that 9B5 and 8C4 target distinct epitopes located at the 5-fold and 3-fold protrusions of CVA16 capsids, respectively, and exhibit differential binding preference to three forms of naturally occurring CVA16 particles. Moreover, 9B5 and 8C4 are compatible in formulating an antibody cocktail which displays the ability to prevent virus escape seen with individual MAbs. Together, our work elucidates the functional and structural basis of CVA16 antibody-mediated neutralization and protection, providing important information for design and development of effective CVA16 vaccines and antibody therapies.
External linksNat Commun / PubMed:36543790 / PubMed Central
MethodsEM (single particle)
Resolution2.9 - 3.8 Å
Structure data

33670

7y7m

EMDB-33670, PDB-7y7m:
The structure of coxsackievirus A16 mature virion in complex with Fab 8C4
Method: EM (single particle) / Resolution: 3.05 Å

33941

7yms

EMDB-33941, PDB-7yms:
Cryo-EM structure of Coxsackievirus A16 in complex with a neutralizing antibody 9B5
Method: EM (single particle) / Resolution: 2.9 Å

34054

7yrf

EMDB-34054, PDB-7yrf:
Cryo-EM structure of compact CA16 empty particle in complex with a neutralizing antibody 8C4
Method: EM (single particle) / Resolution: 2.91 Å

34062

7yrh

EMDB-34062, PDB-7yrh:
Cryo-EM structure of compact coxsackievirus A16 empty particle in complex with a neutralizing antibody 9B5
Method: EM (single particle) / Resolution: 3.35 Å

34118

7yv2

EMDB-34118, PDB-7yv2:
Cryo-EM structure of expanded coxsackievirus A16 empty particle after incubation with 8C4 antibody
Method: EM (single particle) / Resolution: 3.36 Å

34119

7yv7

EMDB-34119, PDB-7yv7:
Cryo-EM structure of expanded coxsackievirus A16 empty particle in complex with antibody 9B5
Method: EM (single particle) / Resolution: 3.8 Å

Chemicals

ChemComp-SPH:
SPHINGOSINE / Sphingosine

Source
  • coxsackievirus a16
KeywordsVIRUS / coxsackievirus A16 / antibody / cryo-EM / STRUCTURAL PROTEIN / antibody 9B5 / antibody 8C4

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