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Title | In situ structure of the red algal phycobilisome-PSII-PSI-LHC megacomplex. |
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Journal, issue, pages | Nature, Vol. 616, Issue 7955, Page 199-206, Year 2023 |
Publish date | Mar 15, 2023 |
Authors | Xin You / Xing Zhang / Jing Cheng / Yanan Xiao / Jianfei Ma / Shan Sun / Xinzheng Zhang / Hong-Wei Wang / Sen-Fang Sui / |
PubMed Abstract | In oxygenic photosynthetic organisms, light energy is captured by antenna systems and transferred to photosystem II (PSII) and photosystem I (PSI) to drive photosynthesis. The antenna systems of red ...In oxygenic photosynthetic organisms, light energy is captured by antenna systems and transferred to photosystem II (PSII) and photosystem I (PSI) to drive photosynthesis. The antenna systems of red algae consist of soluble phycobilisomes (PBSs) and transmembrane light-harvesting complexes (LHCs). Excitation energy transfer pathways from PBS to photosystems remain unclear owing to the lack of structural information. Here we present in situ structures of PBS-PSII-PSI-LHC megacomplexes from the red alga Porphyridium purpureum at near-atomic resolution using cryogenic electron tomography and in situ single-particle analysis, providing interaction details between PBS, PSII and PSI. The structures reveal several unidentified and incomplete proteins and their roles in the assembly of the megacomplex, as well as a huge and sophisticated pigment network. This work provides a solid structural basis for unravelling the mechanisms of PBS-PSII-PSI-LHC megacomplex assembly, efficient energy transfer from PBS to the two photosystems, and regulation of energy distribution between PSII and PSI. |
External links | Nature / PubMed:36922595 |
Methods | EM (single particle) |
Resolution | 3.3 - 6.3 Å |
Structure data | EMDB-33558, PDB-7y1a: EMDB-33605, PDB-7y4l: EMDB-33618, PDB-7y5e: EMDB-33658, PDB-7y7a: EMDB-33669: In situ double-PBS-PSII-PSI-LHCs megacomplex from red alga Porphyridium purpureum. |
Chemicals | ChemComp-PEB: ChemComp-PUB: ChemComp-CYC: ChemComp-BCR: ChemComp-CL: ChemComp-CLA: ChemComp-LHG: ChemComp-OEX: ChemComp-FE: ChemComp-PHO: ChemComp-LMG: ChemComp-BCT: ChemComp-PL9: ChemComp-DGD: ChemComp-HEM: ChemComp-GOL: ChemComp-PQN: ChemComp-SF4: ChemComp-3XQ: ChemComp-FES: ChemComp-BGC: ChemComp-ZEX: ChemComp-HOH: |
Source |
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Keywords | PHOTOSYNTHESIS / Lateral hexamer / PBS / complex / Double-PBS-PSII-PSI-LHCs |