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TitleMultiple nanocages of a cyanophage small heat shock protein with icosahedral and octahedral symmetries.
Journal, issue, pagesSci Rep, Vol. 11, Issue 1, Page 21023, Year 2021
Publish dateOct 25, 2021
AuthorsSreeparna Biswas / Priyanka Garg / Somnath Dutta / Kaza Suguna /
PubMed AbstractThe structures of a cyanophage small heat shock protein (sHSP) were determined as octahedrons of 24-mers and 48-mers and as icosahedrons of 60-mers. An N-terminal deletion construct of an 18 kDa ...The structures of a cyanophage small heat shock protein (sHSP) were determined as octahedrons of 24-mers and 48-mers and as icosahedrons of 60-mers. An N-terminal deletion construct of an 18 kDa sHSP of Synechococcus sp. phage S-ShM2 crystallized as a 24-mer and its structure was determined at a resolution of 7 Å. The negative stain electron microscopy (EM) images showed that the full-length protein is a mixture of a major population of larger and a minor population of smaller cage-like particles. Their structures have been determined by electron cryomicroscopy 3D image reconstruction at a resolution of 8 Å. The larger particles are 60-mers with icosahedral symmetry and the smaller ones are 48-mers with octahedral symmetry. These structures are the first of the viral/phage origin and the 60-mer is the largest and the first icosahedral assembly to be reported for sHSPs.
External linksSci Rep / PubMed:34697325 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution7 - 8.0 Å
Structure data

EMDB-32016:
Small heat shock protein (60-mer) from Synechococcus phage S-ShM2
Method: EM (single particle) / Resolution: 8.0 Å

EMDB-32017:
Small heat shock protein (48-mer) from Synechococcus phage S-ShM2
Method: EM (single particle) / Resolution: 7.7 Å

PDB-7ck4:
Structural and functional analysis of small heat shock protein from Synechococcus phage S-ShM2
Method: X-RAY DIFFRACTION / Resolution: 7.0 Å

Source
  • synechococcus phage s-shm2 (virus)
KeywordsCHAPERONE / small heat shock protein / oligomers / nanocage / chaperone activity.

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