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-Structure paper
タイトル | Multiple nanocages of a cyanophage small heat shock protein with icosahedral and octahedral symmetries. |
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ジャーナル・号・ページ | Sci Rep, Vol. 11, Issue 1, Page 21023, Year 2021 |
掲載日 | 2021年10月25日 |
![]() | Sreeparna Biswas / Priyanka Garg / Somnath Dutta / Kaza Suguna / ![]() |
PubMed 要旨 | The structures of a cyanophage small heat shock protein (sHSP) were determined as octahedrons of 24-mers and 48-mers and as icosahedrons of 60-mers. An N-terminal deletion construct of an 18 kDa ...The structures of a cyanophage small heat shock protein (sHSP) were determined as octahedrons of 24-mers and 48-mers and as icosahedrons of 60-mers. An N-terminal deletion construct of an 18 kDa sHSP of Synechococcus sp. phage S-ShM2 crystallized as a 24-mer and its structure was determined at a resolution of 7 Å. The negative stain electron microscopy (EM) images showed that the full-length protein is a mixture of a major population of larger and a minor population of smaller cage-like particles. Their structures have been determined by electron cryomicroscopy 3D image reconstruction at a resolution of 8 Å. The larger particles are 60-mers with icosahedral symmetry and the smaller ones are 48-mers with octahedral symmetry. These structures are the first of the viral/phage origin and the 60-mer is the largest and the first icosahedral assembly to be reported for sHSPs. |
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手法 | EM (単粒子) / X線回折 |
解像度 | 7 - 8.0 Å |
構造データ | ![]() EMDB-32016: ![]() EMDB-32017: ![]() PDB-7ck4: |
由来 |
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![]() | CHAPERONE / small heat shock protein / oligomers / nanocage / chaperone activity. |