+Search query
-Structure paper
Title | Multiple nanocages of a cyanophage small heat shock protein with icosahedral and octahedral symmetries. |
---|---|
Journal, issue, pages | Sci Rep, Vol. 11, Issue 1, Page 21023, Year 2021 |
Publish date | Oct 25, 2021 |
![]() | Sreeparna Biswas / Priyanka Garg / Somnath Dutta / Kaza Suguna / ![]() |
PubMed Abstract | The structures of a cyanophage small heat shock protein (sHSP) were determined as octahedrons of 24-mers and 48-mers and as icosahedrons of 60-mers. An N-terminal deletion construct of an 18 kDa ...The structures of a cyanophage small heat shock protein (sHSP) were determined as octahedrons of 24-mers and 48-mers and as icosahedrons of 60-mers. An N-terminal deletion construct of an 18 kDa sHSP of Synechococcus sp. phage S-ShM2 crystallized as a 24-mer and its structure was determined at a resolution of 7 Å. The negative stain electron microscopy (EM) images showed that the full-length protein is a mixture of a major population of larger and a minor population of smaller cage-like particles. Their structures have been determined by electron cryomicroscopy 3D image reconstruction at a resolution of 8 Å. The larger particles are 60-mers with icosahedral symmetry and the smaller ones are 48-mers with octahedral symmetry. These structures are the first of the viral/phage origin and the 60-mer is the largest and the first icosahedral assembly to be reported for sHSPs. |
![]() | ![]() ![]() ![]() |
Methods | EM (single particle) / X-ray diffraction |
Resolution | 7 - 8.0 Å |
Structure data | ![]() EMDB-32016: ![]() EMDB-32017: ![]() PDB-7ck4: |
Source |
|
![]() | CHAPERONE / small heat shock protein / oligomers / nanocage / chaperone activity. |