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Structure paper

TitleAssembly mechanism of the pleomorphic immature poxvirus scaffold.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 1704, Year 2022
Publish dateMar 31, 2022
AuthorsJaekyung Hyun / Hideyuki Matsunami / Tae Gyun Kim / Matthias Wolf /
PubMed AbstractIn Vaccinia virus (VACV), the prototype poxvirus, scaffold protein D13 forms a honeycomb-like lattice on the viral membrane that results in formation of the pleomorphic immature virion (IV). The ...In Vaccinia virus (VACV), the prototype poxvirus, scaffold protein D13 forms a honeycomb-like lattice on the viral membrane that results in formation of the pleomorphic immature virion (IV). The structure of D13 is similar to those of major capsid proteins that readily form icosahedral capsids in nucleocytoplasmic large DNA viruses (NCLDVs). However, the detailed assembly mechanism of the nonicosahedral poxvirus scaffold has never been understood. Here we show the cryo-EM structures of the D13 trimer and scaffold intermediates produced in vitro. The structures reveal that the displacement of the short N-terminal α-helix is critical for initiation of D13 self-assembly. The continuous curvature of the IV is mediated by electrostatic interactions that induce torsion between trimers. The assembly mechanism explains the semiordered capsid-like arrangement of D13 that is distinct from icosahedral NCLDVs. Our structures explain how a single protein can self-assemble into different capsid morphologies and represent a local exception to the universal Caspar-Klug theory of quasi-equivalence.
External linksNat Commun / PubMed:35361762 / PubMed Central
MethodsEM (single particle) / EM (helical sym.)
Resolution2.25 - 7.33 Å
Structure data

31949

7vfd

EMDB-31949, PDB-7vfd:
Cryo-EM structure of Vaccinia virus scaffolding protein D13
Method: EM (single particle) / Resolution: 2.25 Å

31950

7vfe

EMDB-31950, PDB-7vfe:
Cryo-EM structure of Vaccinia virus scaffolding protein D13 with N-terminal polyhistidine tag
Method: EM (single particle) / Resolution: 2.63 Å

31951

7vff

EMDB-31951, PDB-7vff:
Cryo-EM structure of Vaccinia virus scaffolding protein D13 with N-terminal 17 residue truncation
Method: EM (single particle) / Resolution: 4.1 Å

31952

7vfg

EMDB-31952, PDB-7vfg:
Cryo-EM structure of Vaccinia virus scaffolding protein D13 trimer doublet
Method: EM (single particle) / Resolution: 3.87 Å

EMDB-31953:
Cryo-EM structure of Vaccinia virus scaffolding protein D13 tubular assembly
Method: EM (helical sym.) / Resolution: 7.33 Å

31954

7vfh

EMDB-31954, PDB-7vfh:
Cryo-EM structure of Vaccinia virus scaffolding protein D13 trimer sextet
Method: EM (single particle) / Resolution: 3.9 Å

Chemicals

ChemComp-HOH:
WATER / Water

Source
  • Vaccinia virus WR
  • vaccinia virus (strain western reserve)
KeywordsVIRAL PROTEIN / Scaffold / capsid / double-jelly-roll

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