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TitleThree structural solutions for bacterial adhesion pilus stability and superelasticity.
Journal, issue, pagesStructure, Vol. 31, Issue 5, Page 529-540.e7, Year 2023
Publish dateMay 4, 2023
AuthorsMatthew H Doran / Joseph L Baker / Tobias Dahlberg / Magnus Andersson / Esther Bullitt /
PubMed AbstractBacterial adhesion pili are key virulence factors that mediate host-pathogen interactions in diverse epithelial environments. Deploying a multimodal approach, we probed the structural basis ...Bacterial adhesion pili are key virulence factors that mediate host-pathogen interactions in diverse epithelial environments. Deploying a multimodal approach, we probed the structural basis underpinning the biophysical properties of pili originating from enterotoxigenic (ETEC) and uropathogenic bacteria. Using cryo-electron microscopy we solved the structures of three vaccine target pili from ETEC bacteria, CFA/I, CS17, and CS20. Pairing these and previous pilus structures with force spectroscopy and steered molecular dynamics simulations, we find a strong correlation between subunit-subunit interaction energies and the force required for pilus unwinding, irrespective of genetic similarity. Pili integrate three structural solutions for stabilizing their assemblies: layer-to-layer interactions, N-terminal interactions to distant subunits, and extended loop interactions from adjacent subunits. Tuning of these structural solutions alters the biophysical properties of pili and promotes the superelastic behavior that is essential for sustained bacterial attachment.
External linksStructure / PubMed:37001523 / PubMed Central
MethodsEM (helical sym.)
Resolution3.2 - 3.4 Å
Structure data

28150

8ehr

EMDB-28150, PDB-8ehr:
Cryo-EM reconstruction of the CFA/I bacterial adhesion pili
Method: EM (helical sym.) / Resolution: 3.2 Å

28151

8ehs

EMDB-28151, PDB-8ehs:
Cryo-EM reconstruction of the CS17 bacterial adhesion pili
Method: EM (helical sym.) / Resolution: 3.3 Å

28152

8eht

EMDB-28152, PDB-8eht:
Cryo-EM reconstruction of the CS20 bacterial adhesion pili
Method: EM (helical sym.) / Resolution: 3.4 Å

Source
  • escherichia coli (E. coli)
KeywordsCELL ADHESION / enterotoxigenic / adhesion pili / superelastic / helical reconstruction

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