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タイトル | Three structural solutions for bacterial adhesion pilus stability and superelasticity. |
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ジャーナル・号・ページ | Structure, Vol. 31, Issue 5, Page 529-540.e7, Year 2023 |
掲載日 | 2023年5月4日 |
著者 | Matthew H Doran / Joseph L Baker / Tobias Dahlberg / Magnus Andersson / Esther Bullitt / |
PubMed 要旨 | Bacterial adhesion pili are key virulence factors that mediate host-pathogen interactions in diverse epithelial environments. Deploying a multimodal approach, we probed the structural basis ...Bacterial adhesion pili are key virulence factors that mediate host-pathogen interactions in diverse epithelial environments. Deploying a multimodal approach, we probed the structural basis underpinning the biophysical properties of pili originating from enterotoxigenic (ETEC) and uropathogenic bacteria. Using cryo-electron microscopy we solved the structures of three vaccine target pili from ETEC bacteria, CFA/I, CS17, and CS20. Pairing these and previous pilus structures with force spectroscopy and steered molecular dynamics simulations, we find a strong correlation between subunit-subunit interaction energies and the force required for pilus unwinding, irrespective of genetic similarity. Pili integrate three structural solutions for stabilizing their assemblies: layer-to-layer interactions, N-terminal interactions to distant subunits, and extended loop interactions from adjacent subunits. Tuning of these structural solutions alters the biophysical properties of pili and promotes the superelastic behavior that is essential for sustained bacterial attachment. |
リンク | Structure / PubMed:37001523 / PubMed Central |
手法 | EM (らせん対称) |
解像度 | 3.2 - 3.4 Å |
構造データ | EMDB-28150, PDB-8ehr: EMDB-28151, PDB-8ehs: EMDB-28152, PDB-8eht: |
由来 |
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キーワード | CELL ADHESION / enterotoxigenic / adhesion pili / superelastic / helical reconstruction |