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TitleStructural characterization of an envelope-associated adeno-associated virus type 2 capsid.
Journal, issue, pagesVirology, Vol. 565, Page 22-28, Year 2022
Publish dateJan 2, 2022
AuthorsJoshua A Hull / Mario Mietzsch / Paul Chipman / David Strugatsky / Robert McKenna /
PubMed AbstractAdeno-associated virus (AAV) are classified as non-enveloped ssDNA viruses. However, AAV capsids embedded within exosomes have been observed, and it has been suggested that the AAV membrane ...Adeno-associated virus (AAV) are classified as non-enveloped ssDNA viruses. However, AAV capsids embedded within exosomes have been observed, and it has been suggested that the AAV membrane associated accessory protein (MAAP) may play a role in envelope-associated AAV (EA-AAV) capsid formation. Here, we observed and selected sufficient homogeneous EA-AAV capsids of AAV2, produced using the Sf9 baculoviral expression system, to determine the cryo-electron microscopy (cryo-EM) structure at 3.14 Å resolution. The reconstructed map confirmed that the EA-AAV capsid, showed no significant structural variation compared to the non-envelope capsid. In addition, the Sf9 expression system used implies the notion that MAAP may enhance exosome AAV encapsulation. Furthermore, we speculate that these EA-AAV capsids may have therapeutic benefits over the currently used non-envelope AAV capsids, with advantages in immune evasion and/or improved infectivity.
External linksVirology / PubMed:34638006 / PubMed Central
MethodsEM (single particle)
Resolution2.43 - 3.14 Å
Structure data

24718

7rwl

EMDB-24718, PDB-7rwl:
Envelope-associated Adeno-associated virus serotype 2
Method: EM (single particle) / Resolution: 3.14 Å

24719

7rwt

EMDB-24719, PDB-7rwt:
Adeno-associated virus type 2
Method: EM (single particle) / Resolution: 2.43 Å

Source
  • adeno-associated dependoparvovirus a
KeywordsVIRUS / exo-AAV / exosome-associated adeno-associated virus / EA-AAV / Envelope-associated adeno-associated virus / Adeno-associated virus type 2

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