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TitleIdentification and architecture of a putative secretion tube across mycobacterial outer envelope.
Journal, issue, pagesSci Adv, Vol. 7, Issue 34, Year 2021
Publish dateAug 20, 2021
AuthorsXiaoying Cai / Lei Liu / Chunhong Qiu / Chongzheng Wen / Yao He / Yanxiang Cui / Siyu Li / Xuan Zhang / Longhua Zhang / Changlin Tian / Lijun Bi / Z Hong Zhou / Weimin Gong /
PubMed AbstractTuberculosis-causing mycobacteria have thick cell-wall and capsule layers that are formed from complex structures. Protein secretion across these barriers depends on a specialized protein secretion ...Tuberculosis-causing mycobacteria have thick cell-wall and capsule layers that are formed from complex structures. Protein secretion across these barriers depends on a specialized protein secretion system, but none has been reported. We show that Rv3705c and its homologous MSMEG_6251 in are tube-forming proteins in the mycobacterial envelope (TiME). Crystallographic and cryo-EM structures of these two proteins show that both proteins form rotationally symmetric rings. Two layers of TiME rings pack together in a tail-to-tail manner into a ring-shaped complex, which, in turn, stacks together to form tubes. TiME was detected mainly in the cell wall and capsule. Knocking out the TiME gene markedly decreased the amount of secreted protein in the culture medium, and expression of this gene in knocked-out strain partially restored the level of secreted protein. Our structure and functional data thus suggest that TiME forms a protein transport tube across the mycobacterial outer envelope.
External linksSci Adv / PubMed:34417177 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution1.55 - 6.2 Å
Structure data

EMDB-22705:
Cryo-EM reconstruction of mtTiME-FL (Rv3705c-FL) in Mycobacterium tuberculosis with D8 symmetry
Method: EM (single particle) / Resolution: 4.4 Å

EMDB-22706:
Cryo-EM reconstruction of mtTiME-FL (Rv3705c-FL) in Mycobacterium tuberculosis with C1 symmetry
Method: EM (single particle) / Resolution: 6.2 Å

PDB-7cu8:
Crystal structure of the soluble domain of TiME protein from Mycobacterium tuberculosis
Method: X-RAY DIFFRACTION / Resolution: 3.3 Å

PDB-7cu9:
Crystal structure of the soluble domain of TiME protein from Mycobacterium smegmatis
Method: X-RAY DIFFRACTION / Resolution: 1.55 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-MG:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

Source
  • Mycobacterium tuberculosis (bacteria)
  • mycobacterium tuberculosis (strain atcc 25618 / h37rv) (bacteria)
  • mycolicibacterium smegmatis (strain atcc 700084 / mc(2)155) (bacteria)
KeywordsPROTEIN TRANSPORT / tubular protein / envelope-spanning channel / potential drug target

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