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-Structure paper
タイトル | Identification and architecture of a putative secretion tube across mycobacterial outer envelope. |
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ジャーナル・号・ページ | Sci Adv, Vol. 7, Issue 34, Year 2021 |
掲載日 | 2021年8月20日 |
著者 | Xiaoying Cai / Lei Liu / Chunhong Qiu / Chongzheng Wen / Yao He / Yanxiang Cui / Siyu Li / Xuan Zhang / Longhua Zhang / Changlin Tian / Lijun Bi / Z Hong Zhou / Weimin Gong / |
PubMed 要旨 | Tuberculosis-causing mycobacteria have thick cell-wall and capsule layers that are formed from complex structures. Protein secretion across these barriers depends on a specialized protein secretion ...Tuberculosis-causing mycobacteria have thick cell-wall and capsule layers that are formed from complex structures. Protein secretion across these barriers depends on a specialized protein secretion system, but none has been reported. We show that Rv3705c and its homologous MSMEG_6251 in are tube-forming proteins in the mycobacterial envelope (TiME). Crystallographic and cryo-EM structures of these two proteins show that both proteins form rotationally symmetric rings. Two layers of TiME rings pack together in a tail-to-tail manner into a ring-shaped complex, which, in turn, stacks together to form tubes. TiME was detected mainly in the cell wall and capsule. Knocking out the TiME gene markedly decreased the amount of secreted protein in the culture medium, and expression of this gene in knocked-out strain partially restored the level of secreted protein. Our structure and functional data thus suggest that TiME forms a protein transport tube across the mycobacterial outer envelope. |
リンク | Sci Adv / PubMed:34417177 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 1.55 - 6.2 Å |
構造データ | EMDB-22705: EMDB-22706: PDB-7cu8: PDB-7cu9: |
化合物 | ChemComp-SO4: ChemComp-MG: ChemComp-GOL: ChemComp-HOH: |
由来 |
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キーワード | PROTEIN TRANSPORT / tubular protein / envelope-spanning channel / potential drug target |