Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Inhibition mechanisms of AcrF9, AcrF8, and AcrF6 against type I-F CRISPR-Cas complex revealed by cryo-EM.
Journal, issue, pages	Proc Natl Acad Sci U S A, Vol. 117, Issue 13, Page 7176-7182, Year 2020
Publish date	Mar 31, 2020
Authors	Kaiming Zhang / Shuo Wang / Shanshan Li / Yuwei Zhu / Grigore D Pintilie / Tung-Chung Mou / Michael F Schmid / Zhiwei Huang / Wah Chiu /
PubMed Abstract	Prokaryotes and viruses have fought a long battle against each other. Prokaryotes use CRISPR-Cas-mediated adaptive immunity, while conversely, viruses evolve multiple anti-CRISPR (Acr) proteins to ...Prokaryotes and viruses have fought a long battle against each other. Prokaryotes use CRISPR-Cas-mediated adaptive immunity, while conversely, viruses evolve multiple anti-CRISPR (Acr) proteins to defeat these CRISPR-Cas systems. The type I-F CRISPR-Cas system in requires the crRNA-guided surveillance complex (Csy complex) to recognize the invading DNA. Although some Acr proteins against the Csy complex have been reported, other relevant Acr proteins still need studies to understand their mechanisms. Here, we obtain three structures of previously unresolved Acr proteins (AcrF9, AcrF8, and AcrF6) bound to the Csy complex using electron cryo-microscopy (cryo-EM), with resolution at 2.57 Å, 3.42 Å, and 3.15 Å, respectively. The 2.57-Å structure reveals fine details for each molecular component within the Csy complex as well as the direct and water-mediated interactions between proteins and CRISPR RNA (crRNA). Our structures also show unambiguously how these Acr proteins bind differently to the Csy complex. AcrF9 binds to key DNA-binding sites on the Csy spiral backbone. AcrF6 binds at the junction between Cas7.6f and Cas8f, which is critical for DNA duplex splitting. AcrF8 binds to a distinct position on the Csy spiral backbone and forms interactions with crRNA, which has not been seen in other Acr proteins against the Csy complex. Our structure-guided mutagenesis and biochemistry experiments further support the anti-CRISPR mechanisms of these Acr proteins. Our findings support the convergent consequence of inhibiting degradation of invading DNA by these Acr proteins, albeit with different modes of interactions with the type I-F CRISPR-Cas system.
External links	Proc Natl Acad Sci U S A / PubMed:32170016 / PubMed Central
Methods	EM (single particle)
Resolution	2.57 - 3.42 Å
Structure data	21358 6vqv EMDB-21358, PDB-6vqv: Type I-F CRISPR-Csy complex with its inhibitor AcrF9 Method: EM (single particle) / Resolution: 2.57 Å 21359 6vqw EMDB-21359, PDB-6vqw: Type I-F CRISPR-Csy complex with its inhibitor AcrF8 Method: EM (single particle) / Resolution: 3.42 Å 21360 6vqx EMDB-21360, PDB-6vqx: Type I-F CRISPR-Csy complex with its inhibitor AcrF6 Method: EM (single particle) / Resolution: 3.15 Å
Source	pseudomonas aeruginosa (bacteria)
Keywords	RNA BINDING PROTEIN/RNA/INHIBITOR / CRISPR / Type I-F / Csy / AcrF9 / anti-CRISPR / RNA BINDING PROTEIN-RNA-INHIBITOR complex / AcrF8 / AcrF6