EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mapping Polyclonal Antibody Responses in Non-human Primates Vaccinated with HIV Env Trimer Subunit Vaccines.
ジャーナル・号・ページ	Cell Rep, Vol. 30, Issue 11, Page 3755-33765.e7, Year 2020
掲載日	2020年3月17日
著者	Bartek Nogal / Matteo Bianchi / Christopher A Cottrell / Robert N Kirchdoerfer / Leigh M Sewall / Hannah L Turner / Fangzhu Zhao / Devin Sok / Dennis R Burton / Lars Hangartner / Andrew B Ward /
PubMed 要旨	Rational immunogen design aims to focus antibody responses to vulnerable sites on primary antigens. Given the size of these antigens, there is, however, potential for eliciting unwanted, off-target ...Rational immunogen design aims to focus antibody responses to vulnerable sites on primary antigens. Given the size of these antigens, there is, however, potential for eliciting unwanted, off-target responses. Here, we use our electron microscopy polyclonal epitope mapping approach to describe the antibody specificities elicited by immunization of non-human primates with soluble HIV envelope trimers and subsequent repeated viral challenge. An increased diversity of epitopes recognized and the approach angle by which these antibodies bind constitute a hallmark of the humoral response in most protected animals. We also show that fusion peptide-specific antibodies are likely responsible for some neutralization breadth. Moreover, cryoelectron microscopy (cryo-EM) analysis of a fully protected animal reveals a high degree of clonality within a subset of putatively neutralizing antibodies, enabling a detailed molecular description of the antibody paratope. Our results provide important insights into the immune response against a vaccine candidate that entered into clinical trials in 2019.
リンク	Cell Rep / PubMed:32187547 / PubMed Central
手法	EM (単粒子)
解像度	3.87 - 32.8 Å
構造データ	20396 6v0r EMDB-20396: Cryo-EM Reconstruction of Polyclonal Serum Fab From a Protected Rhesus Macaque Complexed with BG505 SOSIP.664 PDB-6v0r: BG505 SOSIP.664 Trimer 手法: EM (単粒子) / 解像度: 3.87 Å EMDB-20399: Unimmunized control rhesus macaque BZ05 (challenged with SHIV) serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 16.4 Å EMDB-20400: Unimmunized control rhesus macaque BZ11 (challenged with SHIV) serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 26.8 Å EMDB-20401: Unimmunized control rhesus macaque BZ13 (challenged with SHIV) serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 32.8 Å EMDB-20402: BG505-immunized rhesus macaque 12-060 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 22.7 Å EMDB-20403: BG505-immunized rhesus macaque 12-065 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 24.6 Å EMDB-20404: BG505-immunized rhesus macaque OQ7 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 26.8 Å EMDB-20405: BG505-immunized rhesus macaque 12-153 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 24.6 Å EMDB-20409: BG505-immunized rhesus macaque 12M-248 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 28.7 Å EMDB-20410: BG505-immunized rhesus macaque 12-149 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 29.5 Å EMDB-20415: BG505-immunized rhesus macaque 12-143 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 16.4 Å EMDB-20423: BG505-immunized rhesus macaque 12-137 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 12.8 Å EMDB-20424: BG505-immunized rhesus macaque 12M-169 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 18.45 Å EMDB-20425: BG505-immunized rhesus macaque 11M-088 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 24.6 Å EMDB-20426: BG505-immunized rhesus macaque 12-046 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 15.6 Å EMDB-20427: BG505-immunized rhesus macaque 4O9 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 16.4 Å EMDB-20428: BG505-immunized rhesus macaque 12-137 wk 10 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 19.7 Å EMDB-20429: BG505-immunized rhesus macaque 11M-088 wk 12 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 17.4 Å EMDB-20884: Negative-stain EM map of BG505-immunized rhesus macaque 12-084 wk 28 serum fab complexed with BG505 SOSIP trimer 手法: EM (単粒子) / 解像度: 24.6 Å EMDB-20885: Negative-stain EM map of BG505-immunized rhesus macaque 12-046 wk 26 serum fab complexed with 25710 手法: EM (単粒子) / 解像度: 29.5 Å EMDB-20886: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 4O9 wk 26 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 24.5 Å EMDB-20887: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 4O9 wk 26 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 24.5 Å EMDB-20888: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 4O9 wk 10 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 26.5 Å EMDB-20889: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 4O9 wk 10 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 26.5 Å EMDB-20890: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 4O9 wk 10 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 24.5 Å EMDB-20891: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 11M-088 wk 28 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 24.5 Å EMDB-20892: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 11M-088 wk 28 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 26.5 Å EMDB-20893: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 11M-088 wk 12 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 26.5 Å EMDB-20894: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 11M-088 wk 12 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 25.5 Å EMDB-20895: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 11M-088 wk 12 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 26.5 Å EMDB-20896: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12-137 wk 28 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 24.0 Å EMDB-20897: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12-137 wk 28 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 25.0 Å EMDB-20898: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12-137 wk 28 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 26.5 Å EMDB-20899: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12-137 wk 10 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 24.5 Å EMDB-20921: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12-046 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 24.5 Å EMDB-20936: Negative-stain EM map of BG505-immunized rhesus macaque 12-137 wk 26 serum fab complexed with 398F1 SOSIP.664 手法: EM (単粒子) / 解像度: 22.7 Å EMDB-20937: Negative-stain EM map of BG505-immunized rhesus macaque 12-046 wk 26 serum fab complexed with 398F1 SOSIP.664 手法: EM (単粒子) / 解像度: 26.8 Å EMDB-20938: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12-065 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 26.5 Å EMDB-20939: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque OQ7 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 27.0 Å EMDB-20940: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque OQ7 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 27.0 Å EMDB-20941: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12-143 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 22.5 Å EMDB-20942: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12-046 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 22.5 Å EMDB-20943: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12-046 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 25.0 Å EMDB-20944: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12-137 wk 10 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 22.5 Å EMDB-20946: BG505-immunized rhesus macaque 12-137 wk 28 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 24.5 Å EMDB-20970: BG505-immunized rhesus macaque 11M088 wk 28 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 24.6 Å EMDB-20971: BG505-immunized rhesus macaque 4O9 wk 10 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 26.8 Å EMDB-20972: BG505-immunized rhesus macaque 4O9 wk 26 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 24.6 Å EMDB-20973: Negative-stain EM map from 3D sorting of unimmunized control rhesus macaque BZ05 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 26.0 Å EMDB-20974: Negative-stain EM map from 3D sorting of unimmunized control rhesus macaque BZ05 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 26.5 Å EMDB-20975: Negative-stain EM map from 3D sorting of unimmunized control rhesus macaque BZ05 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 24.7 Å EMDB-20976: Negative-stain EM map from 3D sorting of unimmunized control rhesus macaque BZ11 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 24.0 Å EMDB-20977: Negative-stain EM map from 3D sorting of unimmunized control rhesus macaque BZ11 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 25.0 Å EMDB-20978: Negative-stain EM map from 3D sorting of unimmunized control rhesus macaque BZ11 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 26.7 Å EMDB-20979: Negative-stain EM map from 3D sorting of unimmunized control rhesus macaque BZ13 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 24.6 Å EMDB-20980: Negative-stain EM map from 3D sorting of unimmunized control rhesus macaque BZ13 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 26.5 Å EMDB-20981: Negative-stain EM map from 3D sorting of unimmunized control rhesus macaque BZ13 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 24.5 Å EMDB-20982: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12-060 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 24.5 Å EMDB-20983: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12-060 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 22.5 Å EMDB-20984: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12-065 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 26.0 Å EMDB-20985: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12-065 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 24.5 Å EMDB-20987: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12-143 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 26.5 Å EMDB-20988: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12-143 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 22.5 Å EMDB-20989: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 12-137 wk 0 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 22.0 Å EMDB-20990: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 11M-088 wk 28 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 24.5 Å EMDB-20991: Negative-stain EM map from 3D sorting of BG505-immunized rhesus macaque 4O9 wk 26 serum fab complexed with BG505 SOSIP.664 trimer 手法: EM (単粒子) / 解像度: 24.5 Å EMDB-20992: Negative-stain EM map of BG505-immunized rhesus macaque 12-084 wk 26 serum fab complexed with 398F1 SOSIP.664 手法: EM (単粒子) / 解像度: 22.5 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン
由来	Macaca mulatta (アカゲザル) human immunodeficiency virus 1 (ヒト免疫不全ウイルス)
キーワード	VIRAL PROTEIN / antibody / HIV / glycoprotein / polyclonal / non-human primate / rhesus macaque