EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural asymmetry governs the assembly and GTPase activity of McrBC restriction complexes.
ジャーナル・号・ページ	Nat Commun, Vol. 11, Issue 1, Page 5907, Year 2020
掲載日	2020年11月20日
著者	Yiming Niu / Hiroshi Suzuki / Christopher J Hosford / Thomas Walz / Joshua S Chappie /
PubMed 要旨	McrBC complexes are motor-driven nucleases functioning in bacterial self-defense by cleaving foreign DNA. The GTP-specific AAA + protein McrB powers translocation along DNA and its hydrolysis ...McrBC complexes are motor-driven nucleases functioning in bacterial self-defense by cleaving foreign DNA. The GTP-specific AAA + protein McrB powers translocation along DNA and its hydrolysis activity is stimulated by its partner nuclease McrC. Here, we report cryo-EM structures of Thermococcus gammatolerans McrB and McrBC, and E. coli McrBC. The McrB hexamers, containing the necessary catalytic machinery for basal GTP hydrolysis, are intrinsically asymmetric. This asymmetry directs McrC binding so that it engages a single active site, where it then uses an arginine/lysine-mediated hydrogen-bonding network to reposition the asparagine in the McrB signature motif for optimal catalytic function. While the two McrBC complexes use different DNA-binding domains, these contribute to the same general GTP-recognition mechanism employed by all G proteins. Asymmetry also induces distinct inter-subunit interactions around the ring, suggesting a coordinated and directional GTP-hydrolysis cycle. Our data provide insights into the conserved molecular mechanisms governing McrB family AAA + motors.
リンク	Nat Commun / PubMed:33219217 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.44 - 4.26 Å
構造データ	20865 6ut4 EMDB-20865, PDB-6ut4: Cryo-EM structure of the asymmetric AAA+ domain hexamer from Thermococcus gammatolerans McrB 手法: EM (単粒子) / 解像度: 3.1 Å 20866 6ut5 EMDB-20866, PDB-6ut5: Cryo-EM structure of the Thermococcus gammatolerans McrBC complex 手法: EM (単粒子) / 解像度: 2.44 Å 20867 6ut6 EMDB-20867, PDB-6ut6: Cryo-EM structure of the Escherichia coli McrBC complex 手法: EM (単粒子) / 解像度: 3.28 Å 20868 6ut7 EMDB-20868: Full map of the tetradecameric assembly of Thermococcus gammatolerans McrB AAA+ hexamers with bound McrC in C2 symmetry PDB-6ut7: Fitted model for the tetradecameric assembly of Thermococcus gammatolerans McrB AAA+ hexamers with bound McrC 手法: EM (単粒子) / 解像度: 4.26 Å EMDB-20869: Body 1 for multi-body refinement of the McrB-AAA+ and McrC complex from Thermococcus gammatolerans 手法: EM (単粒子) / 解像度: 3.95 Å EMDB-20870: Body 2 for multi-body refinement of the McrB-AAA+ and McrC complex from Thermococcus gammatolerans 手法: EM (単粒子) / 解像度: 4.1 Å 20871 6ut8 EMDB-20871: Combined map of the half-complex from tetradecameric assembly of Thermococcus gammatolerans McrB AAA+ hexamers with bound McrC PDB-6ut8: Refined half-complex from tetradecameric assembly of Thermococcus gammatolerans McrB AAA+ hexamers with bound McrC 手法: EM (単粒子) / 解像度: 3.68 Å PDB-6ut3: X-ray structure of Thermococcus gammatolerans McrB AAA+ domain hexamer in P21 symmetry 手法: X-RAY DIFFRACTION / 解像度: 2.95 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-GSP: 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE / GTP-γ-S ChemComp-HOH: WATER / 水 ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子*YM / グアノシン二リン酸
由来	thermococcus gammatolerans (古細菌) escherichia coli (strain k12) (大腸菌) thermococcus gammatolerans (strain dsm 15229 / jcm 11827 / ej3) (古細菌)
キーワード	DNA BINDING PROTEIN (DNA結合タンパク質) / AAA protein / GTPase (GTPアーゼ) / Methylation-dependent restriction / Endonuclease (エンドヌクレアーゼ)