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TitleThe inner junction complex of the cilia is an interaction hub that involves tubulin post-translational modifications.
Journal, issue, pagesElife, Vol. 9, Year 2020
Publish dateJan 17, 2020
AuthorsAhmad Abdelzaher Zaki Khalifa / Muneyoshi Ichikawa / Daniel Dai / Shintaroh Kubo / Corbin Steven Black / Katya Peri / Thomas S McAlear / Simon Veyron / Shun Kai Yang / Javier Vargas / Susanne Bechstedt / Jean-François Trempe / Khanh Huy Bui /
PubMed AbstractMicrotubules are cytoskeletal structures involved in stability, transport and organization in the cell. The building blocks, the α- and β-tubulin heterodimers, form protofilaments that associate ...Microtubules are cytoskeletal structures involved in stability, transport and organization in the cell. The building blocks, the α- and β-tubulin heterodimers, form protofilaments that associate laterally into the hollow microtubule. Microtubule also exists as highly stable doublet microtubules in the cilia where stability is needed for ciliary beating and function. The doublet microtubule maintains its stability through interactions at its inner and outer junctions where its A- and B-tubules meet. Here, using cryo-electron microscopy, bioinformatics and mass spectrometry of the doublets of and , we identified two new inner junction proteins, FAP276 and FAP106, and an inner junction-associated protein, FAP126, thus presenting the complete answer to the inner junction identity and localization. Our structural study of the doublets shows that the inner junction serves as an interaction hub that involves tubulin post-translational modifications. These interactions contribute to the stability of the doublet and hence, normal ciliary motility.
External linksElife / PubMed:31951202 / PubMed Central
MethodsEM (single particle)
Resolution3.6 - 4.57 Å
Structure data

20855

6ve7

EMDB-20855: 48-nm repeat unit of the doublet microtubule from Chlamydomonas reinhardtii
PDB-6ve7: The inner junction complex of Chlamydomonas reinhardtii doublet microtubule
Method: EM (single particle) / Resolution: 4.57 Å

EMDB-20856:
16-nm repeat of the doublet microtubule from Tetrahymena thermophila
Method: EM (single particle) / Resolution: 3.6 Å

20858

6ve7

EMDB-20858, PDB-6ve7:
The inner junction complex of Chlamydomonas reinhardtii doublet microtubule
Method: EM (single particle) / Resolution: 3.6 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-MG:
Unknown entry

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

ChemComp-TA1:
TAXOL / medication, chemotherapy*YM / Paclitaxel

Source
  • chlamydomonas reinhardtii (plant)
  • Tetrahymena thermophila (eukaryote)
KeywordsSTRUCTURAL PROTEIN / cilia / doublet / axoneme / microtubule inner protein

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