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TitleStructural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes.
Journal, issue, pagesStructure, Vol. 27, Issue 12, Page 1782-11797.e7, Year 2019
Publish dateDec 3, 2019
AuthorsSanchaita Das / Andrew W Malaby / Agata Nawrotek / Wenhua Zhang / Mahel Zeghouf / Sarah Maslen / Mark Skehel / Srinivas Chakravarthy / Thomas C Irving / Osman Bilsel / Jacqueline Cherfils / David G Lambright /
PubMed AbstractMembrane dynamic processes require Arf GTPase activation by guanine nucleotide exchange factors (GEFs) with a Sec7 domain. Cytohesin family Arf GEFs function in signaling and cell migration through ...Membrane dynamic processes require Arf GTPase activation by guanine nucleotide exchange factors (GEFs) with a Sec7 domain. Cytohesin family Arf GEFs function in signaling and cell migration through Arf GTPase activation on the plasma membrane and endosomes. In this study, the structural organization of two cytohesins (Grp1 and ARNO) was investigated in solution by size exclusion-small angle X-ray scattering and negative stain-electron microscopy and on membranes by dynamic light scattering, hydrogen-deuterium exchange-mass spectrometry and guanosine diphosphate (GDP)/guanosine triphosphate (GTP) exchange assays. The results suggest that cytohesins form elongated dimers with a central coiled coil and membrane-binding pleckstrin-homology (PH) domains at opposite ends. The dimers display significant conformational heterogeneity, with a preference for compact to intermediate conformations. Phosphoinositide-dependent membrane recruitment is mediated by one PH domain at a time and alters the conformational dynamics to prime allosteric activation by Arf-GTP. A structural model for membrane targeting and allosteric activation of full-length cytohesin dimers is discussed.
External linksStructure / PubMed:31601460 / PubMed Central
MethodsEM (single particle)
Resolution53.0 Å
Structure data

20628

6u3e

EMDB-20628: Volume 1 for truncated dimeric Cytohesin-3 (Grp1; amino acids 14-399)
PDB-6u3e: Best fitting antiparallel model for Volume 1 of truncated dimeric Cytohesin-3 (Grp1; amino acids 14-399)
Method: EM (single particle) / Resolution: 53.0 Å

20629

6u3g

EMDB-20629, PDB-6u3g:
Best fitting antiparallel model for Volume 2 of truncated dimeric Cytohesin-3 (Grp1; amino acids 14-399)
Method: EM (single particle) / Resolution: 53.0 Å

Chemicals

ChemComp-4IP:
INOSITOL-(1,3,4,5)-TETRAKISPHOSPHATE

Source
  • homo sapiens (human)
KeywordsENDOCYTOSIS / Arf GEF / phosphoinositide binding / Sec7 domain / PH domain

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