Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	In-cell structure and snapshots of copia retrotransposons in intact tissue by cryo-ET.
Journal, issue, pages	Cell, Year 2025
Publish date	Mar 5, 2025
Authors	Sven Klumpe / Kirsten A Senti / Florian Beck / Jenny Sachweh / Bernhard Hampoelz / Paolo Ronchi / Viola Oorschot / Marlene Brandstetter / Assa Yeroslaviz / John A G Briggs / Julius Brennecke / Martin Beck / Jürgen M Plitzko /
PubMed Abstract	Long terminal repeat (LTR) retrotransposons belong to the transposable elements (TEs), autonomously replicating genetic elements that integrate into the host's genome. Among animals, Drosophila ...Long terminal repeat (LTR) retrotransposons belong to the transposable elements (TEs), autonomously replicating genetic elements that integrate into the host's genome. Among animals, Drosophila melanogaster serves as an important model organism for TE research and contains several LTR retrotransposons, including the Ty1-copia family, which is evolutionarily related to retroviruses and forms virus-like particles (VLPs). In this study, we use cryo-focused ion beam (FIB) milling and lift-out approaches to visualize copia VLPs in ovarian cells and intact egg chambers, resolving the in situ copia capsid structure to 7.7 Å resolution by cryoelectron tomography (cryo-ET). Although cytoplasmic copia VLPs vary in size, nuclear VLPs are homogeneous and form densely packed clusters, supporting a model in which nuclear import acts as a size selector. Analyzing flies deficient in the TE-suppressing PIWI-interacting RNA (piRNA) pathway, we observe copia's translocation into the nucleus during spermatogenesis. Our findings provide insights into the replication cycle and cellular structural biology of an active LTR retrotransposon.
External links	Cell / PubMed:40049165
Methods	EM (tomography) / EM (subtomogram averaging)
Resolution	7.7 - 35.0 Å
Structure data	EMDB-19643: Tomographic reconstruction of the follicle cell nuclear periphery from high pressure frozen, freeze substituted and resin embedded D. melanogaster egg chamber Method: EM (tomography) EMDB-19644: Tomographic reconstruction of the follicle cell nuclear periphery from high pressure frozen, freeze substituted and resin embedded D. melanogaster egg chamber Method: EM (tomography) EMDB-19645: Tomographic reconstruction of the follicle cell nuclear periphery from high pressure frozen, freeze substituted and resin embedded D. melanogaster egg chamber Method: EM (tomography) EMDB-19646: Tomographic reconstruction of the follicle cell nuclear periphery from high pressure frozen, freeze substituted and resin embedded D. melanogaster egg chamber Method: EM (tomography) EMDB-19647: Tomographic reconstruction of nuclear copia VLP clusters in D. melanogaster ovarian somatic cells Method: EM (tomography) EMDB-19648: Tomogram of the nuclear periphery of a follicle cell from lift-out experiments on D. melanogaster egg chambers Method: EM (tomography) EMDB-19649: In situ copia VLP cluster subtomogram averaging, C1-C1 contact map Method: EM (subtomogram averaging) / Resolution: 35.0 Å EMDB-19650: In situ copia VLP cluster subtomogram averaging, C1-C5 contact map Method: EM (subtomogram averaging) / Resolution: 35.0 Å EMDB-19651: In situ copia VLP cluster subtomogram averaging, C5-C5 contact map Method: EM (subtomogram averaging) / Resolution: 35.0 Å 19708 8s41 EMDB-19708, PDB-8s41: The structure of the copia retrotransposon icosahedral capsid (T=9) Method: EM (subtomogram averaging) / Resolution: 7.7 Å
Source	drosophila melanogaster (fruit fly)
Keywords	VIRUS LIKE PARTICLE / Tomography / Drosophila / melanogaster / follicle cell / copia / Ty1 / retrotransposon / LTR