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Structure paper

TitleFilamentID reveals the composition and function of metabolic enzyme polymers during gametogenesis
Journal, issue, pagesCell(Cambridge,Mass. ), Year 2024
Publish dateFeb 5, 2024 (structure data deposition date)
AuthorsHugener J / Xu J / Wettstein R / Ioannidi L / Velikov D / Wollweber F / Henggeler A / Matos J / Pilhofer M
External linksCell(Cambridge,Mass. ) / Search PubMed
MethodsEM (helical sym.) / EM (single particle) / EM (subtomogram averaging) / EM (tomography)
Resolution3.5 - 44.0 Å
Structure data

EMDB-19548, PDB-8rwj:
cryoEM structure of Acs1 filament determined by FilamentID
Method: EM (helical sym.) / Resolution: 3.5 Å

EMDB-19549, PDB-8rwk:
cryoEM structure of the central Ald4 filament determined by FilamentID
Method: EM (single particle) / Resolution: 3.8 Å

EMDB-19550: cryoEM structure of purified Acs1 filament from meiotic yeast cells
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-19551: cryo sub-tomogram average of Acs1 filament from spread meiotic yeast spheroplasts
Method: EM (subtomogram averaging) / Resolution: 18.3 Å

EMDB-19552: cryo sub-tomogram average of Ald4 filaments from purified meiotic yeast mitochondria
Method: EM (subtomogram averaging) / Resolution: 44.0 Å

EMDB-19553: cryo-tomogram of FIB-milled meiotic yeast cell containing filaments in mitochondria
Method: EM (tomography)

EMDB-19554: cryo-tomogram of FIB-milled meiotic yeast cell containing filaments in the nucleus
Method: EM (tomography)

EMDB-19555: cryo-tomogram of FIB-milled meiotic yeast cell containing filaments in the cytoplasm
Method: EM (tomography)

EMDB-19556: cryo-tomogram of purified meiotic yeast mitochondrion containing Ald4 filaments
Method: EM (tomography)

EMDB-19557: cryo-tomogram of spread meiotic yeast spheroplast containing Acs1 filaments
Method: EM (tomography)

EMDB-19558: cryo-tomogram of spread starved yeast spheroplast containing filaments
Method: EM (tomography)

Chemicals

ChemComp-6R9:
[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] ethanoate

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

Source
  • Saccharomyces cerevisiae (brewer's yeast)
  • saccharomyces cerevisiae sk1 (yeast)
KeywordsCYTOSOLIC PROTEIN / metabolic enzyme / filament / cryoEM

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