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TitleInfluence of Amino Acid Substitutions in Capsid Proteins of Coxsackievirus B5 on Free Chlorine and Thermal Inactivation.
Journal, issue, pagesEnviron Sci Technol, Vol. 58, Issue 12, Page 5279-5289, Year 2024
Publish dateMar 26, 2024
AuthorsShotaro Torii / Jérôme Gouttenoire / Kiruthika Kumar / Aleksandar Antanasijevic / Tamar Kohn /
PubMed AbstractThe sensitivity of enteroviruses to disinfectants varies among genetically similar variants and coincides with amino acid changes in capsid proteins, although the effect of individual substitutions ...The sensitivity of enteroviruses to disinfectants varies among genetically similar variants and coincides with amino acid changes in capsid proteins, although the effect of individual substitutions remains unknown. Here, we employed reverse genetics to investigate how amino acid substitutions in coxsackievirus B5 (CVB5) capsid proteins affect the virus' sensitivity to free chlorine and heat treatment. Of ten amino acid changes observed in CVB5 variants with free chlorine resistance, none significantly reduced the chlorine sensitivity, indicating a minor role of the capsid composition in chlorine sensitivity of CVB5. Conversely, a subset of these amino acid changes located at the C-terminal region of viral protein 1 led to reduced heat sensitivity. Cryo-electron microscopy revealed that these changes affect the assembly of intermediate viral states (altered and empty particles), suggesting that the mechanism for reduced heat sensitivity could be related to improved molecular packing of CVB5, resulting in greater stability or altered dynamics of virus uncoating during infection.
External linksEnviron Sci Technol / PubMed:38488515 / PubMed Central
MethodsEM (single particle)
Resolution2.6 - 3.6 Å
Structure data

18942

8r5x

EMDB-18942, PDB-8r5x:
Structure of coxsackievirus B5 capsid (mutant CVB5F.cas.genogroupB) - F particle
Method: EM (single particle) / Resolution: 3.6 Å

18943

8r5y

EMDB-18943, PDB-8r5y:
Structure of coxsackievirus B5 capsid (mutant CVB5F.cas.genogroupB) - A particle
Method: EM (single particle) / Resolution: 2.7 Å

18944

8r5z

EMDB-18944, PDB-8r5z:
Structure of coxsackievirus B5 capsid (mutant CVB5F.cas.genogroupB) - E particle
Method: EM (single particle) / Resolution: 2.6 Å

Chemicals

ChemComp-PLM:
PALMITIC ACID / Palmitic acid

Source
  • coxsackievirus b5
KeywordsVIRUS / Enterovirus / coxsackievirus / thermostable / mutant

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