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TitleMorphofunctional changes at the active zone during synaptic vesicle exocytosis.
Journal, issue, pagesEMBO Rep, Vol. 24, Issue 5, Page e55719, Year 2023
Publish dateMay 4, 2023
AuthorsJulika Radecke / Raphaela Seeger / Anna Kádková / Ulrike Laugks / Amin Khosrozadeh / Kenneth N Goldie / Vladan Lučić / Jakob B Sørensen / Benoît Zuber /
PubMed AbstractSynaptic vesicle (SV) fusion with the plasma membrane (PM) proceeds through intermediate steps that remain poorly resolved. The effect of persistent high or low exocytosis activity on intermediate ...Synaptic vesicle (SV) fusion with the plasma membrane (PM) proceeds through intermediate steps that remain poorly resolved. The effect of persistent high or low exocytosis activity on intermediate steps remains unknown. Using spray-mixing plunge-freezing cryo-electron tomography we observe events following synaptic stimulation at nanometer resolution in near-native samples. Our data suggest that during the stage that immediately follows stimulation, termed early fusion, PM and SV membrane curvature changes to establish a point contact. The next stage-late fusion-shows fusion pore opening and SV collapse. During early fusion, proximal tethered SVs form additional tethers with the PM and increase the inter-SV connector number. In the late-fusion stage, PM-proximal SVs lose their interconnections, allowing them to move toward the PM. Two SNAP-25 mutations, one arresting and one disinhibiting spontaneous release, cause connector loss. The disinhibiting mutation causes loss of membrane-proximal multiple-tethered SVs. Overall, tether formation and connector dissolution are triggered by stimulation and respond to spontaneous fusion rate manipulation. These morphological observations likely correspond to SV transition from one functional pool to another.
External linksEMBO Rep / PubMed:36876590 / PubMed Central
MethodsEM (tomography)
Structure data

EMDB-18728: Rat synaptosome
Method: EM (tomography)

EMDB-18744: Stimulated rat synaptosome.
Method: EM (tomography)

EMDB-18746: wild type neuronal synapse
Method: EM (tomography)

EMDB-18748: SNAP-25-4E neuronal synapse
Method: EM (tomography)

EMDB-18749: SNAP-25-4K neuronal synapse
Method: EM (tomography)

Source
  • Rattus norvegicus (Norway rat)
  • Mus musculus (house mouse)

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