EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insights into the cross-exon to cross-intron spliceosome switch.
ジャーナル・号・ページ	Nature, Year 2024
掲載日	2024年5月22日
著者	Zhenwei Zhang / Vinay Kumar / Olexandr Dybkov / Cindy L Will / Jiayun Zhong / Sebastian E J Ludwig / Henning Urlaub / Berthold Kastner / Holger Stark / Reinhard Lührmann /
PubMed 要旨	Early spliceosome assembly can occur through an intron-defined pathway, whereby U1 and U2 small nuclear ribonucleoprotein particles (snRNPs) assemble across the intron. Alternatively, it can occur ...Early spliceosome assembly can occur through an intron-defined pathway, whereby U1 and U2 small nuclear ribonucleoprotein particles (snRNPs) assemble across the intron. Alternatively, it can occur through an exon-defined pathway, whereby U2 binds the branch site located upstream of the defined exon and U1 snRNP interacts with the 5' splice site located directly downstream of it. The U4/U6.U5 tri-snRNP subsequently binds to produce a cross-intron (CI) or cross-exon (CE) pre-B complex, which is then converted to the spliceosomal B complex. Exon definition promotes the splicing of upstream introns and plays a key part in alternative splicing regulation. However, the three-dimensional structure of exon-defined spliceosomal complexes and the molecular mechanism of the conversion from a CE-organized to a CI-organized spliceosome, a pre-requisite for splicing catalysis, remain poorly understood. Here cryo-electron microscopy analyses of human CE pre-B complex and B-like complexes reveal extensive structural similarities with their CI counterparts. The results indicate that the CE and CI spliceosome assembly pathways converge already at the pre-B stage. Add-back experiments using purified CE pre-B complexes, coupled with cryo-electron microscopy, elucidate the order of the extensive remodelling events that accompany the formation of B complexes and B-like complexes. The molecular triggers and roles of B-specific proteins in these rearrangements are also identified. We show that CE pre-B complexes can productively bind in trans to a U1 snRNP-bound 5' splice site. Together, our studies provide new mechanistic insights into the CE to CI switch during spliceosome assembly and its effect on pre-mRNA splice site pairing at this stage.
リンク	Nature / PubMed:38778104
手法	EM (単粒子)
解像度	3.1 - 9.6 Å
構造データ	18542 8qoz EMDB-18542, PDB-8qoz: Cryo-EM Structure of Pre-B+5'ss+ATPgammaS Complex (core part) 手法: EM (単粒子) / 解像度: 3.1 Å 18544 8qp8 EMDB-18544, PDB-8qp8: Cryo-EM Structure of Pre-B Complex (core part) 手法: EM (単粒子) / 解像度: 3.5 Å 18545 8qp9 EMDB-18545, PDB-8qp9: Cryo-EM Structure of Pre-B+AMPPNP Complex (core part) 手法: EM (単粒子) / 解像度: 4.1 Å 18546 8qpa EMDB-18546, PDB-8qpa: Cryo-EM Structure of Pre-B+5'ssLNG Complex (core part) 手法: EM (単粒子) / 解像度: 3.7 Å 18547 8qpb EMDB-18547, PDB-8qpb: Cryo-EM Structure of Pre-B+ATP Complex (core part) 手法: EM (単粒子) / 解像度: 3.7 Å 18548 8qpe EMDB-18548, PDB-8qpe: Cryo-EM Structure of Pre-B-like Complex (core part) 手法: EM (単粒子) / 解像度: 3.1 Å 18555 8qpk EMDB-18555, PDB-8qpk: Cryo-EM Structure of Pre-B+5'ss Complex (core part) 手法: EM (単粒子) / 解像度: 4.2 Å 18718 8qxd EMDB-18718, PDB-8qxd: Cryo-EM structure of the cross-exon pre-B complex 手法: EM (単粒子) / 解像度: 9.6 Å 18781 8qzs EMDB-18781, PDB-8qzs: Cryo-EM structure of the cross-exon B-like complex 手法: EM (単粒子) / 解像度: 4.1 Å 18786 8r08 EMDB-18786, PDB-8r08: Cryo-EM structure of the cross-exon pre-B+AMPPNP complex 手法: EM (単粒子) / 解像度: 6.1 Å 18787 8r09 EMDB-18787, PDB-8r09: Cryo-EM structure of the cross-exon pre-B+5'ss+ATPgammaS complex 手法: EM (単粒子) / 解像度: 4.3 Å 18788 8r0a EMDB-18788, PDB-8r0a: Cryo-EM structure of the cross-exon pre-B+5'ss complex 手法: EM (単粒子) / 解像度: 5.8 Å 18789 8r0b EMDB-18789, PDB-8r0b: Cryo-EM structure of the cross-exon pre-B+ATP complex 手法: EM (単粒子) / 解像度: 4.4 Å 19349 8rm5 EMDB-19349, PDB-8rm5: Cryo-EM structure of the cross-exon pre-B+5'ssLNG+ATPyS complex 手法: EM (単粒子) / 解像度: 6.9 Å
化合物	ChemComp-IHP: INOSITOL HEXAKISPHOSPHATE / フィチン酸 / フィチン酸
由来	homo sapiens (ヒト)
キーワード	SPLICING / spliceosome (スプライセオソーム)