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TitleStructural basis for the subunit assembly of the anaphase-promoting complex.
Journal, issue, pagesNature, Vol. 470, Issue 7333, Page 227-232, Year 2011
Publish dateFeb 10, 2011
AuthorsAnne Schreiber / Florian Stengel / Ziguo Zhang / Radoslav I Enchev / Eric H Kong / Edward P Morris / Carol V Robinson / Paula C A da Fonseca / David Barford /
PubMed AbstractThe anaphase-promoting complex or cyclosome (APC/C) is an unusually large E3 ubiquitin ligase responsible for regulating defined cell cycle transitions. Information on how its 13 constituent proteins ...The anaphase-promoting complex or cyclosome (APC/C) is an unusually large E3 ubiquitin ligase responsible for regulating defined cell cycle transitions. Information on how its 13 constituent proteins are assembled, and how they interact with co-activators, substrates and regulatory proteins is limited. Here, we describe a recombinant expression system that allows the reconstitution of holo APC/C and its sub-complexes that, when combined with electron microscopy, mass spectrometry and docking of crystallographic and homology-derived coordinates, provides a precise definition of the organization and structure of all essential APC/C subunits, resulting in a pseudo-atomic model for 70% of the APC/C. A lattice-like appearance of the APC/C is generated by multiple repeat motifs of most APC/C subunits. Three conserved tetratricopeptide repeat (TPR) subunits (Cdc16, Cdc23 and Cdc27) share related superhelical homo-dimeric architectures that assemble to generate a quasi-symmetrical structure. Our structure explains how this TPR sub-complex, together with additional scaffolding subunits (Apc1, Apc4 and Apc5), coordinate the juxtaposition of the catalytic and substrate recognition module (Apc2, Apc11 and Apc10 (also known as Doc1)), and TPR-phosphorylation sites, relative to co-activator, regulatory proteins and substrates.
External linksNature / PubMed:21307936
MethodsEM (single particle)
Structure data

EMDB-1841:
Structural basis for the subunit assembly of the anaphase promoting complex
Method: EM (single particle)

EMDB-1842:
Structural basis for the subunit assembly of the anaphase promoting complex
Method: EM (single particle)

EMDB-1843:
Structural basis for the subunit assembly of the anaphase promoting complex
Method: EM (single particle)

EMDB-1844:
Structural basis for the subunit assembly of the anaphase promoting complex
Method: EM (single particle)

EMDB-1845:
Structural basis for the subunit assembly of the anaphase promoting complex
Method: EM (single particle)

Source
  • Saccharomyces cerevisiae (brewer's yeast)

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