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-Structure paper
Title | Structural specializations of the sperm tail. |
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Journal, issue, pages | Cell, Vol. 186, Issue 13, Page 2880-2896.e17, Year 2023 |
Publish date | Jun 13, 2023 |
![]() | Miguel Ricardo Leung / Jianwei Zeng / Xiangli Wang / Marc C Roelofs / Wei Huang / Riccardo Zenezini Chiozzi / Johannes F Hevler / Albert J R Heck / Susan K Dutcher / Alan Brown / Rui Zhang / Tzviya Zeev-Ben-Mordehai / ![]() ![]() |
PubMed Abstract | Sperm motility is crucial to reproductive success in sexually reproducing organisms. Impaired sperm movement causes male infertility, which is increasing globally. Sperm are powered by a microtubule- ...Sperm motility is crucial to reproductive success in sexually reproducing organisms. Impaired sperm movement causes male infertility, which is increasing globally. Sperm are powered by a microtubule-based molecular machine-the axoneme-but it is unclear how axonemal microtubules are ornamented to support motility in diverse fertilization environments. Here, we present high-resolution structures of native axonemal doublet microtubules (DMTs) from sea urchin and bovine sperm, representing external and internal fertilizers. We identify >60 proteins decorating sperm DMTs; at least 15 are sperm associated and 16 are linked to infertility. By comparing DMTs across species and cell types, we define core microtubule inner proteins (MIPs) and analyze evolution of the tektin bundle. We identify conserved axonemal microtubule-associated proteins (MAPs) with unique tubulin-binding modes. Additionally, we identify a testis-specific serine/threonine kinase that links DMTs to outer dense fibers in mammalian sperm. Our study provides structural foundations for understanding sperm evolution, motility, and dysfunction at a molecular level. |
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Methods | EM (single particle) |
Resolution | 3.3 - 4.1 Å |
Structure data | EMDB-17187, PDB-8otz: EMDB-17188, PDB-8ou0: ![]() EMDB-18071: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A-lumen top ![]() EMDB-18072: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A-lumen middle ![]() EMDB-18073: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A-lumen bottom ![]() EMDB-18074: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A01-A02 top ![]() EMDB-18075: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A01-A02 middle ![]() EMDB-18076: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A01-A02 bottom ![]() EMDB-18077: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A02-A04 top ![]() EMDB-18078: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A02-A04 middle ![]() EMDB-18079: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A02-A04 bottom ![]() EMDB-18080: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A04-A06 top ![]() EMDB-18081: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A04-A06 middle ![]() EMDB-18082: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A04-A06 bottom ![]() EMDB-18083: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A07-A08 top ![]() EMDB-18084: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A07-A08 middle ![]() EMDB-18085: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement A07-A08 bottom ![]() EMDB-18086: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Outer Junction top ![]() EMDB-18087: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Outer Junction middle ![]() EMDB-18088: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Outer Junction bottom ![]() EMDB-18089: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Ribbon top ![]() EMDB-18090: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Ribbon middle ![]() EMDB-18091: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Ribbon bottom ![]() EMDB-18092: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Inner Junction top ![]() EMDB-18093: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Inner Junction middle ![]() EMDB-18094: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement Inner Junction bottom ![]() EMDB-18095: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B01-B03 top ![]() EMDB-18096: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B01-B03 middle ![]() EMDB-18097: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B01-B03 bottom ![]() EMDB-18098: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B03-B05 top ![]() EMDB-18099: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B03-B05 middle ![]() EMDB-18100: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B03-B05 bottom ![]() EMDB-18101: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B05-B07 top ![]() EMDB-18102: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B05-B07 middle ![]() EMDB-18103: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B05-B07 bottom ![]() EMDB-18104: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B07-B09 top ![]() EMDB-18105: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B07-B09 middle ![]() EMDB-18106: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, local refinement B07-B09 bottom ![]() EMDB-18107: 48-nm repeat of the native axonemal doublet microtubule from bovine sperm, consensus refinement EMDB-40619: cryo-EM structure of sea urchin sperm doublet microtubule (48-nm periodicity) ![]() EMDB-40620: cryo-EM density map of the 48-nm repeat doublet microtubule with beak from Chlamydomonas ![]() EMDB-40621: cryo-EM density map of the 48-nm repeat doublet microtubule without beak from Chlamydomonas |
Chemicals | ![]() ChemComp-GTP: ![]() ChemComp-MG: ![]() ChemComp-GDP: |
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