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TitleHelical ultrastructure of the L-ENA spore aggregation factor of a Bacillus paranthracis foodborne outbreak strain.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 7514, Year 2024
Publish dateAug 29, 2024
AuthorsMike Sleutel / Ephrem Debebe Zegeye / Ann-Katrin Llarena / Brajabandhu Pradhan / Marcus Fislage / Kristin O'Sullivan / Nani Van Gerven / Marina Aspholm / Han Remaut /
PubMed AbstractIn pathogenic Bacillota, spores can form an infectious particle and can take up a central role in the environmental persistence and dissemination of disease. A poorly understood aspect of spore- ...In pathogenic Bacillota, spores can form an infectious particle and can take up a central role in the environmental persistence and dissemination of disease. A poorly understood aspect of spore-mediated infection is the fibrous structures or 'endospore appendages' (ENAs) that have been seen to decorate the spores of pathogenic Bacilli and Clostridia. Current methodological approaches are opening a window on these long enigmatic structures. Using cryoID, Alphafold modelling and genetic approaches we identify a sub-class of robust ENAs in a Bacillus paranthracis foodborne outbreak strain. We demonstrate that L-ENA are encoded by a rare three-gene cluster (ena3) that contains all components for the self-assembly of ladder-like protein nanofibers of stacked heptameric rings, their anchoring to the exosporium, and their termination in a trimeric 'ruffle' made of a complement C1Q-like BclA paralogue. The role of ENA fibers in spore-spore interaction and the distribution of L-ENA operon as mobile genetic elements in B. cereus s.l. strains suggest that L-ENA fibers may increase the survival, spread and virulence of these strains.
External linksNat Commun / PubMed:39209852 / PubMed Central
MethodsEM (helical sym.)
Resolution3.32 - 5.77 Å
Structure data

EMDB-17579: Ex vivo L-ENA type pili from Bacillus paranthracis endospores
Method: EM (helical sym.) / Resolution: 5.77 Å

17627

8pdz

EMDB-17627, PDB-8pdz:
Recombinant Ena3A L-Type endospore appendages
Method: EM (helical sym.) / Resolution: 3.32 Å

Source
  • bacillus paranthracis (bacteria)
KeywordsPROTEIN FIBRIL / Endospore / Pili / Protein / Fiber / Spore / Appendage / Bacillus paranthracis / recombinant

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