+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-17627 | |||||||||
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Title | Recombinant Ena3A L-Type endospore appendages | |||||||||
Map data | ||||||||||
Sample |
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Keywords | Endospore / Pili / Protein / Fiber / Spore / Appendage / Bacillus paranthracis / PROTEIN FIBRIL / recombinant | |||||||||
Function / homology | Endospore appendages core / Endospore appendages / DUF3992 domain-containing protein Function and homology information | |||||||||
Biological species | Bacillus paranthracis (bacteria) | |||||||||
Method | helical reconstruction / cryo EM / Resolution: 3.32 Å | |||||||||
Authors | Sleutel M / Remaut H | |||||||||
Funding support | Belgium, 1 items
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Citation | Journal: Biorxiv / Year: 2023 Title: A novel class of ultra-stable endospore appendages decorated with collagen-like tip fibrillae Authors: Sleutel M / Zegeye ED / Llarena AK / Pradhan B / Fislage M / O'Sullivan K / Aspholm M / Remaut H | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_17627.map.gz | 50.5 MB | EMDB map data format | |
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Header (meta data) | emd-17627-v30.xml emd-17627.xml | 14 KB 14 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_17627_fsc.xml | 9.9 KB | Display | FSC data file |
Images | emd_17627.png | 71.8 KB | ||
Masks | emd_17627_msk_1.map | 103 MB | Mask map | |
Filedesc metadata | emd-17627.cif.gz | 5.5 KB | ||
Others | emd_17627_half_map_1.map.gz emd_17627_half_map_2.map.gz | 95.6 MB 95.6 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-17627 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-17627 | HTTPS FTP |
-Validation report
Summary document | emd_17627_validation.pdf.gz | 952.5 KB | Display | EMDB validaton report |
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Full document | emd_17627_full_validation.pdf.gz | 952.1 KB | Display | |
Data in XML | emd_17627_validation.xml.gz | 18.4 KB | Display | |
Data in CIF | emd_17627_validation.cif.gz | 23.7 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-17627 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-17627 | HTTPS FTP |
-Related structure data
Related structure data | 8pdzMC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_17627.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 0.764 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
File | emd_17627_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_17627_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_17627_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Recombinant Ena3A L-type endospore appendage
Entire | Name: Recombinant Ena3A L-type endospore appendage |
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Components |
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-Supramolecule #1: Recombinant Ena3A L-type endospore appendage
Supramolecule | Name: Recombinant Ena3A L-type endospore appendage / type: organelle_or_cellular_component / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: Bacillus paranthracis (bacteria) / Strain: NVH 0075-95 / Location in cell: spore surface |
Molecular weight | Theoretical: 18.03 kDa/nm |
-Macromolecule #1: DUF3992 domain-containing protein
Macromolecule | Name: DUF3992 domain-containing protein / type: protein_or_peptide / ID: 1 / Number of copies: 28 / Enantiomer: LEVO |
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Source (natural) | Organism: Bacillus paranthracis (bacteria) |
Molecular weight | Theoretical: 11.58715 KDa |
Recombinant expression | Organism: Escherichia coli (E. coli) |
Sequence | String: MAQIGNCCTE QLCCVNDAVC CTIILDDTGG TALPIWDDAT TFVINGTIMV ENNGTVGVGP TAALTVNGTA VGGFVVAPGE CRSITMNDI NSIAIVGAGT GTSSVKISFS INYKF UniProtKB: DUF3992 domain-containing protein |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | helical reconstruction |
Aggregation state | filament |
-Sample preparation
Buffer | pH: 7 / Component - Formula: miliQ |
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Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | JEOL CRYO ARM 300 |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Number real images: 8528 / Average electron dose: 62.5 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Nominal defocus max: 3.5 µm / Nominal defocus min: 0.5 µm / Nominal magnification: 60000 |