EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural characterization of the full-length Hantaan virus polymerase.
ジャーナル・号・ページ	PLoS Pathog, Vol. 20, Issue 12, Page e1012781, Year 2024
掲載日	2024年12月9日
著者	Jeremy R Keown / Loïc Carrique / Benjamin E Nilsson-Payant / Ervin Fodor / Jonathan M Grimes /
PubMed 要旨	Hantaviridae are a family of segmented negative-sense RNA viruses that contain important human and animal pathogens. Hantaviridae contain a viral RNA-dependent RNA polymerase that replicates and ...Hantaviridae are a family of segmented negative-sense RNA viruses that contain important human and animal pathogens. Hantaviridae contain a viral RNA-dependent RNA polymerase that replicates and transcribes the viral genome. Here we establish the expression and purification of the polymerase from the Old World Hantaan virus and characterise the structure using Cryo-EM. We determine a series of structures at resolutions between 2.7 and 3.3 Å of RNA free polymerase comprising the core, core and endonuclease, and a full-length polymerase. The full-length polymerase structure depicts the location of the cap binding and C-terminal domains which are arranged in a conformation that is incompatible with transcription and in a novel conformation not observed in previous conformations of cap-snatching viral polymerases. We further describe structures with 5' vRNA promoter in the presence and absence of a nucleotide triphosphate. The nucleotide bound structure mimics a replication pre-initiation complex and the nucleotide stabilises the motif E in a conformation distinct from those previously observed. We observe motif E in four distinct conformations including β-sheet, two helical arrangements, and nucleotide primed arrangement. The insights gained here guide future mechanistic studies of both the transcription and replication activities of the hantavirus polymerase and for the development of therapeutic targets.
リンク	PLoS Pathog / PubMed:39652621 / PubMed Central
手法	EM (単粒子)
解像度	2.78 - 3.3 Å
構造データ	17351 8p1j EMDB-17351, PDB-8p1j: Structure of hantaan orthohantavirus (HTNV) polymerase - Apo core 手法: EM (単粒子) / 解像度: 2.78 Å 17352 8p1k EMDB-17352, PDB-8p1k: Structure of hantaan orthohantavirus (HTNV) polymerase - Apo core endonuclease 手法: EM (単粒子) / 解像度: 3.24 Å 17353 8p1l EMDB-17353, PDB-8p1l: Structure of hantaan orthohantavirus (HTNV) polymerase - Apo full length 手法: EM (単粒子) / 解像度: 3.3 Å 17354 8p1m EMDB-17354, PDB-8p1m: Structure of hantaan orthohantavirus (HTNV) polymerase bound to 5'vRNA and NTP Mg 手法: EM (単粒子) / 解像度: 3.23 Å 17355 8p1n EMDB-17355, PDB-8p1n: Structure of hantaan orthohantavirus (HTNV) polymerase bound to 5'vRNA 手法: EM (単粒子) / 解像度: 2.79 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-MG*: Unknown entry / マグネシウムジカチオン
由来	hantaan orthohantavirus (ウイルス)
キーワード	VIRAL PROTEIN / RNA-dependent RNA polymerase / HTNV / Hantaan orthohantavirus / Bunyavirales / Hantaviridae.