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- EMDB-17351: Structure of hantaan orthohantavirus (HTNV) polymerase - Apo core -
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Open data
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Basic information
Entry | ![]() | ||||||||||||
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Title | Structure of hantaan orthohantavirus (HTNV) polymerase - Apo core | ||||||||||||
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![]() | RNA-dependent RNA polymerase / HTNV / Hantaan orthohantavirus / Bunyavirales / Hantaviridae. / VIRAL PROTEIN | ||||||||||||
Function / homology | ![]() RNA-templated viral transcription / negative stranded viral RNA replication / cap snatching / endonuclease activity / Hydrolases; Acting on ester bonds / host cell perinuclear region of cytoplasm / RNA-directed RNA polymerase / RNA-dependent RNA polymerase activity / nucleotide binding / DNA-templated transcription / metal ion binding Similarity search - Function | ||||||||||||
Biological species | ![]() | ||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.78 Å | ||||||||||||
![]() | Keown JR / Carrique L / Grimes JM | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Structure of hantaan orthohantavirus (HTNV) polymerase - Apo core Authors: Keown JR / Carrique L / Grimes JM | ||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 97 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 19.7 KB 19.7 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 11 KB | Display | ![]() |
Images | ![]() | 98.2 KB | ||
Masks | ![]() | 103 MB | ![]() | |
Filedesc metadata | ![]() | 7.4 KB | ||
Others | ![]() ![]() | 95.5 MB 95.5 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8p1jMC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Map
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Voxel size | X=Y=Z: 0.932 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_17351_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_17351_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : RNA-dependent RNA polymerase of Hantaan orthohantavirus (HTNV).
Entire | Name: RNA-dependent RNA polymerase of Hantaan orthohantavirus (HTNV). |
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Components |
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-Supramolecule #1: RNA-dependent RNA polymerase of Hantaan orthohantavirus (HTNV).
Supramolecule | Name: RNA-dependent RNA polymerase of Hantaan orthohantavirus (HTNV). type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 247 KDa |
-Macromolecule #1: RNA-directed RNA polymerase L
Macromolecule | Name: RNA-directed RNA polymerase L / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: RNA-directed RNA polymerase |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 251.806016 KDa |
Recombinant expression | Organism: ![]() ![]() |
Sequence | String: MWSHPQFEKG GGSGGGSGGS SAWSHPQFEK HHHHHHHHEN LYFQGMDKYR EIHNKLKEFS PGTLTAVECI DYLDRLYAVR HDIVDQMIK HDWSDNKDSE EAIGKVLLFA GVPSNIITAL EKKIIPNHPT GKSLKAFFKM TPANYKISGT TIEFVEVTVT A DVDKGIRE ...String: MWSHPQFEKG GGSGGGSGGS SAWSHPQFEK HHHHHHHHEN LYFQGMDKYR EIHNKLKEFS PGTLTAVECI DYLDRLYAVR HDIVDQMIK HDWSDNKDSE EAIGKVLLFA GVPSNIITAL EKKIIPNHPT GKSLKAFFKM TPANYKISGT TIEFVEVTVT A DVDKGIRE KKLKYEAGLT YIEQELHKFF LKGEIPQPYK ITFNVVAVRT DGSNITTQWP SRRNDGVVQY MRLVQAEISY VR EHLIKTE ERAALEAMFN LKFNISTHKS QPYYIPDYKG MEPIGANIED LVDYSKDWLS RARNFSFFEV KGTAVFECFN SNE ANHCQR YPMSRKPRNF LLIQCSLITS YKPATTLSDQ IDSRRACSYI LNLIPDTPAS YLIHDMAYRY INLTREDMIN YYAP RIQFK QTQNVREPGT FKLTSSMLRA ESKAMLDLLN NHKSGEKHGA QIESLNIASH IVQSESVSLI TKILSDLELN ITEPS TQEY STTKHTYVDT VLDKFFQNET QKYLIDVLKK TTAWHIGHLI RDITESLIAH SGLKRSKYWS LHSYNNGNVI LFILPS KSL EVAGSFIRFI TVFRIGPGLV DKDNLDTILI DGDSQWGVSK VMSIDLNRLL ALNIAFEKAL IATATWFQYY TEDQGQF PL QYAIRSVFAN HFLLAICQKM KLCAIFDNLR YLIPAVTSLY SGFPSLIEKL FERPFKSSLE VYIYYNIKSL LVALAQNN K ARFYSKVKLL GLTVDQSTVG ASGVYPSFMS RIVYKHYRSL ISEVTTCFFL FEKGLHGNMN EEAKIHLETV EWALKFREK EEKYGESLVE NGYMMWELRA NAELAEQQLY CQDAIELAAI ELNKVLATKS SVVANSILSK NWEEPYFSQT RNISLKGMSG QVQEDGHLS SSVTIIEAIR YLSNSRHNPS LLKLYEETRE QKAMARIVRK YQRTEADRGF FITTLPTRCR LEIIEDYYDA I AKNISEEY ISYGGEKKIL AIQGALEKAL RWASGESFIE LSNHKFIRMK RKLMYVSADA TKWSPGDNSA KFRRFTSMLH NG LPNNKLK NCVIDALKQV YKTDFFMSRK LRNYIDSMES LDPHIKQFLD FFPDGHHGEV KGNWLQGNLN KCSSLFGVAM SLL FKQVWT NLFPELDCFF EFAHHSDDAL FIYGYLEPVD DGTDWFLFVS QQIQAGHLHW FSVNTEMWKS MFNLHEHILL LGSI KISPK KTTVSPTNAE FLSTFFEGCA VSIPFVKILL GSLSDLPGLG YFDDLAAAQS RCVKALDLGA SPQVAQLAVA LCTSK VERL YGTAPGMVNH PAAYLQVKHT DTPIPLGGNG AMSIMELATA GIGMSDKNLL KRALLGYSHK RQKSMLYILG LFKFLM KLS DETFQHERLG QFSFIGKVQW KIFTPKSEFE FADMYTSKFL ELWSSQHVTY DYIIPKGRDN LLIYLVRKLN DPSIVTA MT MQSPLQLRFR MQAKQHMKVC RLDGEWVTFR EVLAAANSFA ENYSATSQDM DLFQTLTSCT FSKEYAWKDF LNGIHCDV I PTKQVQRAKV ARTFTVREKD QIIQNSIPAV IGYKFAVTVE EMSDVLDTAK FPDSLSVDLK TMKDGVYREL GLDISLPDV MKRIAPMLYK SSKSRVVIVQ GNVEGTAEAI CRYWLKSMSL VKTIRVKPHK EVLQAVSIFN RKEDIGQQKD LAALKLCIEV WRWCKANSA PYRDWFQALW FEDKTFSEWL DRFCRVGVPP IDPEIQCAAL MIADIKGDYS VLQLQANRRA YSGKQYDAYC V QTYNEVTK LYEGDLRVTF NFGLDCARLE IFWDKKAYIL ETSITQKHVL KIMMDEVSKE LIKCGMRFNT EQVQGVRHMV LF KTESGFE WGKPNIPCIV YKNCVLRTSL RTTQAINHKF MITIKDDGLR AIAQHDEDSP RFLLAHAFHT IRDIRYQAVD AVS NVWFIH KGVKLYLNPI ISSGLLENFM KNLPAAIPPA AYSLIMNRAK ISVDLFMFND LLKLINPRNT LDLSGLETTG DEFS TVSSM SSRLWSEEMS LVDDDEELDD EFTIDLQDVD FENIDIEADI EHFLQDESSY TGDLLISTEE TESKKMRGIV KILEP VRLI KSWVSRGLSI EKVYSPVNII LMSRYISKTF NLSTKQVSLL DPYDLTELES IVRGWGECVI DQFESLDREA QNMVVN KGI CPEDVIPDSL FSFRHTMVLL RRLFPQDSIS SFY UniProtKB: RNA-directed RNA polymerase L |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Concentration | 0.5 mg/mL | |||||||||||||||
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Buffer | pH: 7.5 Component:
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Grid | Model: Quantifoil R2/1 / Material: COPPER / Mesh: 200 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 45 sec. | |||||||||||||||
Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 293 K / Instrument: FEI VITROBOT MARK IV |
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Electron microscopy
Microscope | TFS KRIOS |
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Specialist optics | Energy filter - Name: TFS Selectris X / Energy filter - Slit width: 10 eV |
Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.6 µm / Nominal defocus min: 1.4000000000000001 µm |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
-Atomic model buiding 1
Initial model | Chain - Source name: AlphaFold / Chain - Initial model type: in silico model |
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Refinement | Space: REAL / Protocol: FLEXIBLE FIT / Target criteria: Cross-correlation coefficient |
Output model | ![]() PDB-8p1j: |